PMID- 14726389
OWN - NLM
STAT- MEDLINE
DCOM- 20040625
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 103
IP  - 10
DP  - 2004 May 15
TI  - Maturation of dendritic cells abrogates C1q production in vivo and in vitro.
PG  - 3813-20
AB  - Dendritic cells (DCs) and complement are essential components of the innate 
      immune system. Immature DCs (immDCs) and mature DCs (mDCs) can migrate to 
      lymphoid areas inducing, respectively, tolerance and immune responses. Primary 
      deficiency of complement component C1q (C1q) leads to autoimmunity, suggesting a 
      role in the maintenance of tolerance. In the present study, we investigated the 
      production of C1q by immDCs, mDCs, and macrophages. We demonstrated that 
      monocyte-derived and CD34(+)-derived interstitial DCs are a rich source of C1q. 
      C1q produced by immDCs is functionally active in complement activation and 
      binding to apoptotic cells. The production of C1q is completely down-regulated 
      upon DC maturation in vitro. Moreover, we found that DC differentiation in the 
      presence of interferon-alpha (IFN-alpha) accelerated DC maturation and strongly 
      impaired overall C1q production. Finally, we demonstrated the presence, in 
      significant numbers, of DC-SIGN(+)/C1q(+) cells in T-cell areas of tonsils, next 
      to DC-LAMP(+) mDCs lacking C1q. We conclude from these results that immDC, a cell 
      with tolerogenic properties, is a rich source of active C1q in vitro and in vivo, 
      which is down-regulated on maturation. Therefore, immDCs may be considered an 
      additional source of C1q in humans.
FAU - Castellano, Giuseppe
AU  - Castellano G
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, the 
      Netherlands.
FAU - Woltman, Andrea M
AU  - Woltman AM
FAU - Nauta, Alma J
AU  - Nauta AJ
FAU - Roos, Anja
AU  - Roos A
FAU - Trouw, Leendert A
AU  - Trouw LA
FAU - Seelen, Marc A
AU  - Seelen MA
FAU - Schena, Francesco Paolo
AU  - Schena FP
FAU - Daha, Mohamed R
AU  - Daha MR
FAU - van Kooten, Cees
AU  - van Kooten C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040115
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Interferon-alpha)
RN  - 80295-33-6 (Complement C1q)
SB  - IM
MH  - Cell Differentiation/*immunology
MH  - Cells, Cultured
MH  - Complement Activation
MH  - Complement C1q/analysis/*biosynthesis
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Interferon-alpha/pharmacology
MH  - Macrophages/immunology/metabolism
MH  - Palatine Tonsil/cytology
MH  - Phagocytosis
MH  - Time Factors
EDAT- 2004/01/17 05:00
MHDA- 2004/06/26 05:00
CRDT- 2004/01/17 05:00
PHST- 2004/01/17 05:00 [pubmed]
PHST- 2004/06/26 05:00 [medline]
PHST- 2004/01/17 05:00 [entrez]
AID - S0006-4971(20)43668-7 [pii]
AID - 10.1182/blood-2003-09-3046 [doi]
PST - ppublish
SO  - Blood. 2004 May 15;103(10):3813-20. doi: 10.1182/blood-2003-09-3046. Epub 2004 
      Jan 15.