PMID- 14728981
OWN - NLM
STAT- MEDLINE
DCOM- 20040302
LR  - 20190727
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 194
IP  - 1
DP  - 2004 Jan 1
TI  - In vivo conjugation of nasal lavage proteins by hexahydrophthalic anhydride.
PG  - 69-78
AB  - Hexahydrophthalic anhydride (HHPA), an industrially important chemical, is a 
      highly allergenic compound. The aim of this work was to identify proteins in 
      nasal lavage fluid (NLF) that form adducts with HHPA. Such bindings may induce 
      production of specific immunoglobulin E (IgE) or affect physiological mechanisms 
      of the proteins. NLF was obtained from HHPA-exposed volunteers, workers and 
      exposed guinea pigs. HHPA-binding proteins were visualized with immunoblotting 
      using a polyclonal antiserum against HHPA. The proteins were excised from sodium 
      dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, digested 
      with trypsin and identified by tandem mass spectrometry (MS/MS) and database 
      searches. The antiserum was found to be specific for HHPA-bound proteins. In vivo 
      formed HHPA-binding proteins in humans were identified as antileukoproteinase, 
      immunoglobulin G (IgG), immunoglobulin A (IgA), serum albumin and lactoferrin. In 
      addition, several proteins binding to HHPA were found in NLFs from guinea pigs 
      but these could not be identified from database searches. Hypotheses for 
      development of airways diseases by adduction of this allergenic compound to the 
      NLF proteins in humans were established.
FAU - Johannesson, Gunvor
AU  - Johannesson G
AD  - Department of Occupational and Environmental Medicine, Institute of Laboratory 
      Medicine, Lund University Hospital, SE-221 85, Lund, Sweden. 
      gunvor.johannesson@ymed.lu.se
FAU - Lindh, Christian
AU  - Lindh C
FAU - Nielsen, Jorn
AU  - Nielsen J
FAU - Bjork, Birgitta
AU  - Bjork B
FAU - Rosqvist, Seema
AU  - Rosqvist S
FAU - Jonsson, Bo A G
AU  - Jonsson BA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Epoxy Resins)
RN  - 0 (Phthalic Anhydrides)
RN  - 0 (Proteins)
RN  - 37226-48-5 (araldite)
SB  - IM
MH  - Animals
MH  - Epoxy Resins/*metabolism/*pharmacology
MH  - Guinea Pigs
MH  - Humans
MH  - Male
MH  - Nasal Lavage Fluid/*chemistry
MH  - Nasal Mucosa/*drug effects/*metabolism
MH  - Phthalic Anhydrides/*metabolism/*pharmacology
MH  - Protein Binding/drug effects/physiology
MH  - Proteins/*metabolism
EDAT- 2004/01/20 05:00
MHDA- 2004/03/03 05:00
CRDT- 2004/01/20 05:00
PHST- 2004/01/20 05:00 [pubmed]
PHST- 2004/03/03 05:00 [medline]
PHST- 2004/01/20 05:00 [entrez]
AID - S0041008X03004320 [pii]
AID - 10.1016/j.taap.2003.09.001 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2004 Jan 1;194(1):69-78. doi: 10.1016/j.taap.2003.09.001.