PMID- 14729365
OWN - NLM
STAT- MEDLINE
DCOM- 20040304
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 557
IP  - 2
DP  - 2004 Feb 14
TI  - Cytogenetic biomonitoring of a floriculturist population in Italy: micronucleus 
      analysis by fluorescence in situ hybridization (FISH) with an all-chromosome 
      centromeric probe.
PG  - 109-17
AB  - Flower production in greenhouses associated with a heavy use of pesticides is 
      very wide-spread in the western part of the Ligurian region (Italy). The 
      formation of micronuclei in peripheral blood lymphocytes is a valuable 
      cytogenetic biomarker in human populations occupationally exposed to genotoxic 
      compounds. In the present study we investigated the micronucleus frequency in 
      peripheral blood lymphocytes of 52 floriculturists and 24 control subjects by use 
      of the cytokinesis-block methodology associated with fluorescence in situ 
      hybridization with a pan-centromeric probe that allowed to distinguish 
      centromere-positive (C+) and centromere-negative (C-) micronuclei. The comparison 
      between floriculturists and controls did not reveal any statistically significant 
      difference in micronucleus frequency, although an increase was observed with 
      increasing pesticide use, number of genotoxic pesticides used and duration of 
      exposure. An increase in C+ as well as in C- micronuclei and in the percentage of 
      C+ micronuclei with respect to the total number of micronuclei was detected in 
      floriculturists, suggesting a higher contribution of C+ micronuclei in the total 
      number scored. The percentage C+ micronuclei was not related to the duration of 
      exposure or to the number of genotoxic pesticides used, but a higher percentage 
      (66.52% versus 63.78%) was observed in a subgroup of subjects using 
      benzimidazolic compounds, compared with the floriculturist population exposed to 
      a complex pesticide mixture not including benzimidazolics. These results suggest 
      a potential human hazard associated with the exposure to this class of 
      aneuploidy-inducing carcinogens.
FAU - Bolognesi, Claudia
AU  - Bolognesi C
AD  - Environmental Carcinogenesis Unit, National Cancer Research Institute, Largo R. 
      Benzi 10, 16132 Genoa, Italy. claudia.bolognesi@istge.it
FAU - Landini, Eleonora
AU  - Landini E
FAU - Perrone, Emanuela
AU  - Perrone E
FAU - Roggieri, Paola
AU  - Roggieri P
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Pesticides)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Centromere/*genetics
MH  - *Chromosome Aberrations
MH  - Environmental Monitoring/*methods
MH  - Female
MH  - Flowers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - *Micronucleus Tests
MH  - Middle Aged
MH  - *Occupational Exposure
MH  - Pesticides/*adverse effects
EDAT- 2004/01/20 05:00
MHDA- 2004/03/05 05:00
CRDT- 2004/01/20 05:00
PHST- 2004/01/20 05:00 [pubmed]
PHST- 2004/03/05 05:00 [medline]
PHST- 2004/01/20 05:00 [entrez]
AID - S1383571803002869 [pii]
AID - 10.1016/j.mrgentox.2003.09.013 [doi]
PST - ppublish
SO  - Mutat Res. 2004 Feb 14;557(2):109-17. doi: 10.1016/j.mrgentox.2003.09.013.