PMID- 14729430
OWN - NLM
STAT- MEDLINE
DCOM- 20040603
LR  - 20220409
IS  - 0167-5273 (Print)
IS  - 0167-5273 (Linking)
VI  - 93
IP  - 1
DP  - 2004 Jan
TI  - Thymoquinone and Nigella sativa oil protection against methionine-induced 
      hyperhomocysteinemia in rats.
PG  - 19-23
AB  - Although the state of hyperhomocysteinemia (HHcy) appears to be associated with 
      higher risks of coronary, cerebral and peripheral vascular disease as well as 
      with a number of other clinical conditions, the underlying molecular mechanisms 
      are not fully elucidated. There is strong evidence, however, that HHcy could 
      induce a pathogenic state of oxidative stress. The interest in modulating the 
      elevated levels of total homocysteine in HHcy and/or their negative impacts 
      through preventive strategies, particularly through the supplementation with 
      vitamins that may be linked to the homeostasis of homocysteine (folate, vitamin 
      B(12), and vitamin B(6)), has increased in recent years. Here we show that active 
      antioxidant components of the traditionally used black seeds of Nigella sativa 
      plant protect against the development of methionine-induced HHcy and its 
      associated state of oxidative stress. Pretreatment of rats with an oral dose of 
      100 mg/kg of thymoquinone, the main active constituent of the black seed, for 30 
      min and for 1 week almost completely protected against induced HHcy measured 5 h 
      after methionine load (100 mg/kg). Under similar conditions pretreatment with 
      commercial black seed oil (100 microl/kg) for 30 min and for 1 week produced 
      significant and strong protection levels of 74.2 and 94.5%, respectively. Under 
      the state of induced HHcy there were significant increases in the plasma levels 
      of triglycerides, lipid peroxidation, cholesterol and in the activities of 
      glutathione peroxidase and superoxide dismutase. Catalase activity was not 
      affected. The total antioxidant status, however, was significantly depressed. All 
      of these effects were almost totally blocked by prior treatment with thymoquinone 
      or black seed oil. These findings may contribute towards a protective measure 
      utilizing the black seed against the negative impacts of HHcy.
FAU - El-Saleh, Saleh C
AU  - El-Saleh SC
AD  - Department of Medical Microbiology, College of Sciences, King Saud University, 
      P.O. Box 237, Buraidah 81999, Saudi Arabia. assaleh@ksu.edu.sa
FAU - Al-Sagair, Othman A
AU  - Al-Sagair OA
FAU - Al-Khalaf, Maha I
AU  - Al-Khalaf MI
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Int J Cardiol
JT  - International journal of cardiology
JID - 8200291
RN  - 0 (Benzoquinones)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Oils)
RN  - AE28F7PNPL (Methionine)
RN  - O60IE26NUF (thymoquinone)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Benzoquinones/*pharmacology
MH  - Hyperhomocysteinemia/*prevention & control
MH  - Male
MH  - Methionine
MH  - *Nigella sativa
MH  - Oxidative Stress
MH  - *Phytotherapy
MH  - Plant Extracts/*pharmacology
MH  - Plant Oils/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Seeds
EDAT- 2004/01/20 05:00
MHDA- 2004/06/04 05:00
CRDT- 2004/01/20 05:00
PHST- 2004/01/20 05:00 [pubmed]
PHST- 2004/06/04 05:00 [medline]
PHST- 2004/01/20 05:00 [entrez]
AID - S0167527303001086 [pii]
AID - 10.1016/s0167-5273(03)00108-6 [doi]
PST - ppublish
SO  - Int J Cardiol. 2004 Jan;93(1):19-23. doi: 10.1016/s0167-5273(03)00108-6.