PMID- 14734226
OWN - NLM
STAT- MEDLINE
DCOM- 20040305
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 148
IP  - 2
DP  - 2004 Jan 15
TI  - Interphase cytogenetic analysis of clonality in peripheral blood cells from a 
      patient with Down syndrome and acute megakaryoblastic leukemia.
PG  - 141-4
AB  - A combination of fluorescence-activated cell sorting and interphase fluorescence 
      in situ hybridization (FISH) techniques was used to detect a clonal chromosomal 
      marker in blasts, granulocytes, and T and B lymphocytes of the peripheral blood 
      from a patient with Down syndrome and acute megakaryoblastic leukemia (AMKL) 
      associated with trisomy 8 as a karyotypic abnormality. Immunophenotypic studies 
      with flow cytometry showed two populations of leukemic blasts distinguished by 
      their expression of the CD34 antigen. Interphase FISH studies revealed clonal 
      trisomy 8 FISH signals in almost all blast cells, regardless of CD34 expression, 
      as well as in a small subpopulation of granulocytes. Normal chromosome 8 signal 
      patterns were detected in T and B cells and in a great majority of granulocytes. 
      The present study provides evidence for the clonal involvement of leukemic blasts 
      in AMKL of Down syndrome, indicating that a trisomy 8 abnormality may be a 
      primary event in leukemogenesis. The transformation occurs in progenitor cells 
      with limited myeloid differentiation and without involvement of lymphoid lineage 
      cells.
FAU - Chang, Hong
AU  - Chang H
AD  - Princess Margaret Hospital, University Health Network, University of Toronto, 
      4-320 Toronto, Ontario M5G 2M9, Canada. hong.chang@uhn.on.ca
FAU - Li, Dan
AU  - Li D
FAU - Nayar, Rakash
AU  - Nayar R
FAU - Ye, Charles
AU  - Ye C
FAU - Lau, Wendy
AU  - Lau W
FAU - Sutherland, D Robert
AU  - Sutherland DR
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Antigens, CD34/genetics
MH  - Child, Preschool
MH  - Cytogenetic Analysis
MH  - Down Syndrome/*genetics
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Leukemia, Megakaryoblastic, Acute/*genetics
EDAT- 2004/01/22 05:00
MHDA- 2004/03/06 05:00
CRDT- 2004/01/22 05:00
PHST- 2004/01/22 05:00 [pubmed]
PHST- 2004/03/06 05:00 [medline]
PHST- 2004/01/22 05:00 [entrez]
AID - S0165460803002735 [pii]
AID - 10.1016/s0165-4608(03)00273-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Jan 15;148(2):141-4. doi: 
      10.1016/s0165-4608(03)00273-5.