PMID- 14734756
OWN - NLM
STAT- MEDLINE
DCOM- 20040507
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 172
IP  - 3
DP  - 2004 Feb 1
TI  - Human T cell lymphotropic virus type I (HTLV-I)-specific CD4+ T cells: 
      immunodominance hierarchy and preferential infection with HTLV-I.
PG  - 1735-43
AB  - CD4(+) T cells predominate in early lesions in the CNS in the inflammatory 
      disease human lymphotropic T cell virus type I (HTLV-I)-associated 
      myelopathy/tropical spastic paraparesis (HAM/TSP), but the pathogenesis of the 
      disease remains unclear and the HTLV-I-specific CD4(+) T cell response has been 
      little studied. We quantified the IFN-gamma-producing HTLV-I-specific CD4(+) T 
      cells, in patients with HAM/TSP and in asymptomatic carriers with high proviral 
      load, to test two hypotheses: that HAM/TSP patients and asymptomatic HTLV-I 
      carriers with a similar proviral load differ in the immunodominance hierarchy or 
      the total frequency of specific CD4(+) T cells, and that HTLV-I-specific CD4(+) T 
      cells are preferentially infected with HTLV-I. The strongest CD4(+) T cell 
      response in both HAM/TSP patients and asymptomatic carriers was specific to Env. 
      This contrasts with the immunodominance of Tax in the HTLV-I-specific CD8(+) T 
      cell response. The median frequency of HTLV-I-specific IFN-gamma(+) CD4(+) T 
      cells was 25-fold greater in patients with HAM/TSP (p = 0.0023, Mann-Whitney) 
      than in asymptomatic HTLV-I carriers with a similar proviral load. Furthermore, 
      the frequency of CD4(+) T cells infected with HTLV-I (expressing Tax protein) was 
      significantly greater (p = 0.0152, Mann-Whitney) among HTLV-I-specific cells than 
      CMV-specific cells. These data were confirmed by quantitative PCR for HTLV-I DNA. 
      We conclude that the high frequency of specific CD4(+) T cells was associated 
      with the disease HAM/TSP, and did not simply reflect the higher proviral load 
      that is usually found in HAM/TSP patients. Finally, we conclude that 
      HTLV-I-specific CD4(+) T cells are preferentially infected with HTLV-I.
FAU - Goon, Peter K C
AU  - Goon PK
AD  - Department of Immunology, Imperial College London, London, United Kingdom.
FAU - Igakura, Tadahiko
AU  - Igakura T
FAU - Hanon, Emmanuel
AU  - Hanon E
FAU - Mosley, Angelina J
AU  - Mosley AJ
FAU - Barfield, Anna
AU  - Barfield A
FAU - Barnard, Amanda L
AU  - Barnard AL
FAU - Kaftantzi, Lambrini
AU  - Kaftantzi L
FAU - Tanaka, Yuetsu
AU  - Tanaka Y
FAU - Taylor, Graham P
AU  - Taylor GP
FAU - Weber, Jonathan N
AU  - Weber JN
FAU - Bangham, Charles R M
AU  - Bangham CR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Gene Products, env)
RN  - 0 (Gene Products, tax)
RN  - 0 (Immunodominant Epitopes)
SB  - IM
MH  - Amino Acid Sequence
MH  - CD4-Positive T-Lymphocytes/*immunology/pathology/*virology
MH  - CD8-Positive T-Lymphocytes/immunology/virology
MH  - Carrier State/immunology/pathology/virology
MH  - Cytomegalovirus/immunology
MH  - Cytotoxicity, Immunologic
MH  - Epitope Mapping
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Gene Products, env/immunology
MH  - Gene Products, tax/immunology
MH  - HTLV-I Infections/*immunology/pathology/virology
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Humans
MH  - Immunodominant Epitopes/*immunology
MH  - Lymphocyte Count
MH  - Molecular Sequence Data
MH  - Paraparesis, Tropical Spastic/immunology/pathology/virology
MH  - Proviruses/immunology
MH  - Viral Load
EDAT- 2004/01/22 05:00
MHDA- 2004/05/08 05:00
CRDT- 2004/01/22 05:00
PHST- 2004/01/22 05:00 [pubmed]
PHST- 2004/05/08 05:00 [medline]
PHST- 2004/01/22 05:00 [entrez]
AID - 10.4049/jimmunol.172.3.1735 [doi]
PST - ppublish
SO  - J Immunol. 2004 Feb 1;172(3):1735-43. doi: 10.4049/jimmunol.172.3.1735.