PMID- 14735496
OWN - NLM
STAT- MEDLINE
DCOM- 20040920
LR  - 20061115
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 67
IP  - 3
DP  - 2004 Mar
TI  - Human sperm aneuploidy after exposure to pesticides.
PG  - 353-9
AB  - This study examined the effect of paternal environmental exposure to pesticides 
      on the frequency of aneuploidy in human sperm. To determine if the chromosome 
      number in germ cells was altered by paternal exposure, multicolor fluorescence in 
      situ hybridization (FISH) analysis was utilized to measure aneuploidy frequencies 
      in the sperm of 40 men (20 exposed, 20 controls). Samples were coded for "blind 
      analysis" to eliminate scorer bias. Aneuploidy and diploidy frequencies were 
      assessed for chromosomes 13, 21, X, and Y. A minimum of 10,000 sperm was scored 
      per donor per chromosome probe with a total of 809,935 sperm scored. 
      Hybridization efficiency was 99%. There were no significant differences in 
      aneuploidy or diploidy frequencies between exposed and control groups, suggesting 
      that the pesticides did not increase the risk of numerical chromosomal 
      abnormalities in these men.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Smith, Jena L
AU  - Smith JL
AD  - Department of Medical Genetics, Faculty of Medicine, University of Calgary, 
      Calgary, Alberta, Canada.
FAU - Garry, Vincent F
AU  - Garry VF
FAU - Rademaker, Alfred W
AU  - Rademaker AW
FAU - Martin, Renee H
AU  - Martin RH
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Pesticides)
SB  - IM
MH  - *Aneuploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pesticides/*adverse effects
MH  - Spermatozoa/abnormalities/*drug effects
EDAT- 2004/01/22 05:00
MHDA- 2004/09/21 05:00
CRDT- 2004/01/22 05:00
PHST- 2004/01/22 05:00 [pubmed]
PHST- 2004/09/21 05:00 [medline]
PHST- 2004/01/22 05:00 [entrez]
AID - 10.1002/mrd.20022 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2004 Mar;67(3):353-9. doi: 10.1002/mrd.20022.