PMID- 14735564
OWN - NLM
STAT- MEDLINE
DCOM- 20040610
LR  - 20220310
IS  - 0197-8462 (Print)
IS  - 0197-8462 (Linking)
VI  - 25
IP  - 2
DP  - 2004 Feb
TI  - Pulsed electromagnetic field stimulation of bone marrow cells derived from 
      ovariectomized rats affects osteoclast formation and local factor production.
PG  - 134-41
AB  - This study examined the effects of a specific pulsed electromagnetic field (PEMF) 
      stimulation on osteoclast formation in bone marrow cells from ovariectomized rats 
      and to determine if the signal modulates the production of cytokines associated 
      with osteoclast formation. Adult female Wistar rats were subjected to bilateral 
      or sham ovariectomy, and primary bone marrow cells were harvested at 4 days 
      (Subgroup I) and 7 days (Subgroup II) after surgery. Primary bone marrow cells 
      were subsequently placed in chamber slides and set inside solenoids powered by a 
      pulse generator (300 micros, 7.5 Hz) for 1 h per day for 9 days (OVX + PEMF 
      group). Others (INT, SHAM, and OVX groups) were cultured under identical 
      conditions, but no signal was applied. Recruitment and authentication of 
      osteoclast-like cells were evaluated by determining multinuclear, 
      tartrate-resistant acid phosphatase (TRAP) positive cells on day 10 of culture 
      and by pit formation assay, respectively. The PEMF signal caused significant 
      reductions in osteoclast formation in both Subgroups I (-55%) and II (-43%). 
      Tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), and 
      interleukin 6 (IL-6) in OVX + PEMF group of Subgroup I were significantly reduced 
      at 5, 7, and 9 days as compared to OVX group. The results found in this study 
      suggest that osteoclastogenesis can be inhibited by PEMF stimulation, putatively 
      due to a concomitant decrease in local factor production. Bioelectromagnetics 
      25:134-141, 2004.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Chang, Kyle
AU  - Chang K
AD  - Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li, 
      Taiwan, Republic of China.
FAU - Hong-Shong Chang, Walter
AU  - Hong-Shong Chang W
FAU - Yu, Yen-Hsin
AU  - Yu YH
FAU - Shih, Chung
AU  - Shih C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bioelectromagnetics
JT  - Bioelectromagnetics
JID - 8008281
RN  - 0 (Growth Substances)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*physiology/*radiation effects
MH  - Cell Division/radiation effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Radiation
MH  - *Electromagnetic Fields
MH  - Female
MH  - Growth Substances/*metabolism
MH  - Osteoclasts/cytology/*physiology/*radiation effects
MH  - Osteogenesis/*radiation effects
MH  - *Ovariectomy
MH  - Radiation Dosage
MH  - Rats
MH  - Rats, Wistar
EDAT- 2004/01/22 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/01/22 05:00
PHST- 2004/01/22 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/01/22 05:00 [entrez]
AID - 10.1002/bem.10168 [doi]
PST - ppublish
SO  - Bioelectromagnetics. 2004 Feb;25(2):134-41. doi: 10.1002/bem.10168.