PMID- 14736954
OWN - NLM
STAT- MEDLINE
DCOM- 20041005
LR  - 20190513
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 19
IP  - 2
DP  - 2004 Feb
TI  - Renal processing of serum proteins in an albumin-deficient environment: an in 
      vivo study of glomerulonephritis in the Nagase analbuminaemic rat.
PG  - 320-8
AB  - BACKGROUND: Plasma albumin has been considered as important for governing 
      glomerular permselectivity as well as being tubulotoxic in proteinuric states. 
      The purpose of this study was to examine glomerular permselectivity and protein 
      clearance of plasma albumin-deficient Nagase analbuminaemic rats (NAR) in normal 
      and proteinuric states associated with puromycin aminonucleoside nephrosis (PAN) 
      and anti-glomerular basement membrane glomerulonephritis (anti-GBM GN) and to 
      compare the results with those of previous studies using Sprague-Dawley rats. 
      METHODS: Glomerular permselectivity was measured using tritium-labelled 
      polydisperse Ficoll. In vivo fractional clearance (FC) of albumin, transferrin 
      and immunoglobulin G was measured to include both intact and degraded forms of 
      filtered material. Endogenous protein clearance was analysed using 
      two-dimensional electrophoresis in combination with matrix-assisted laser 
      desorption ionization (MALDI) mass spectrometry. RESULTS: FCs of proteins and 
      Ficoll in control NAR were similar to those found in Sprague-Dawley rats. Despite 
      the lack of serum albumin in NAR, proteinuria and morphological changes observed 
      were also similar to those found in Sprague-Dawley rats, with total protein 
      excretion increasing 6-fold in PAN rats and 4-fold in anti-GBM GN rats with 
      respect to controls. Two-dimensional electrophoresis in combination with MALDI 
      mass spectrometry identified the major proteins being excreted as transferrin and 
      a group of mildly acidic proteins in the MW range 40-50 kDa, namely antithrombin 
      III, kininogen, alpha-1-antiproteinase, haemopexin and vitamin D-binding protein. 
      CONCLUSIONS: Both diseases exhibited similar effects to those observed in 
      Sprague-Dawley rats despite the lack of serum albumin, including inhibition of 
      renal protein degradation. The net changes in protein FC, particularly in the 
      range of radii of 36-55 A, could not be accounted for by changes in size 
      selectivity as Ficoll FC was little affected by the disease states. This 
      emphasizes the need to reassess the relative importance of changes in renal 
      tubular handling vs changes in glomerular capillary barrier in proteinuric 
      states. These studies also demonstrate that albumin is not a critical factor in 
      governing glomerular permselectivity or proteinuria.
FAU - Osicka, Tanya M
AU  - Osicka TM
AD  - Endocrine Unit, Austin & Repatriation Medical Centre, Department of Medicine, 
      University of Melbourne, Heidelberg, Victoria, Australia.
FAU - Strong, Kimberley J
AU  - Strong KJ
FAU - Nikolic-Paterson, David J
AU  - Nikolic-Paterson DJ
FAU - Atkins, Robert C
AU  - Atkins RC
FAU - Jerums, George
AU  - Jerums G
FAU - Comper, Wayne D
AU  - Comper WD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Blood Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Transferrin)
RN  - EC 3.2.1.52 (Acetylglucosaminidase)
SB  - IM
MH  - Acetylglucosaminidase
MH  - Albuminuria/*diagnosis/etiology
MH  - Animals
MH  - Blood Protein Electrophoresis
MH  - Blood Proteins/*metabolism
MH  - Chromatography
MH  - Disease Models, Animal
MH  - Female
MH  - Glomerular Filtration Rate
MH  - Glomerulonephritis/complications/*pathology/*physiopathology
MH  - Immunoglobulin G/analysis
MH  - Male
MH  - Probability
MH  - Proteinuria/*diagnosis/etiology
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sensitivity and Specificity
MH  - Transferrin/metabolism
EDAT- 2004/01/23 05:00
MHDA- 2004/10/06 09:00
CRDT- 2004/01/23 05:00
PHST- 2004/01/23 05:00 [pubmed]
PHST- 2004/10/06 09:00 [medline]
PHST- 2004/01/23 05:00 [entrez]
AID - 10.1093/ndt/gfg226 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2004 Feb;19(2):320-8. doi: 10.1093/ndt/gfg226.