PMID- 14737582 OWN - NLM STAT- MEDLINE DCOM- 20040416 LR - 20191210 IS - 0003-2654 (Print) IS - 0003-2654 (Linking) VI - 129 IP - 1 DP - 2004 Jan TI - Sensitive and selective analysis of coenzyme Q10 in human serum by negative APCI LC-MS. PG - 45-50 AB - Two new sensitive and selective LC-MS methods have been developed for the quantification of the total coenzyme Q(10) concentration in human blood serum. The sensitivity of the methods is based on the very efficient formation of the radical anions of CoQ(10)[M(-)[radical dot]] by negative atmospheric pressure ionisation, APCI(-). The mass detection of the [M(-)[radical dot]] ions, m/z= 862.6, was performed either in selective ion monitoring (SIM) or in MS(2) mode (m/z= 862.6 [rightward arrow]m/z= 847.6) using an LCQ-deca ion-trap mass spectrometer. Two standard serum samples with medium (0.73 [micro sign]g ml(-1)) and high (1.96 [micro sign]g ml(-1)) total CoQ(10) concentrations were analysed by LC-APCI(-)-SIM and LC-APCI(-)-MS(2) and the results compared with a HPLC literature procedure with electrochemical detection (ECD). Both the LC-MS methods were shown to be more selective and with comparable or better sensitivity than the HPLC-ECD method. The LC-MS-SIM and LC-MS(2) chromatograms of the medium concentration sample showed CoQ(10) signal to noise ratios of 25 and 625, respectively. In addition, a simple and fast serum pre-treatment procedure was developed, in which the serum CoQ(10)H(2) content was quantitatively oxidised quantitatively to CoQ(10) in less than 15 min by 1,4-benzoquinone. FAU - Hansen, Gregers AU - Hansen G AD - Department of Life Sciences and Chemistry, Roskilde University, DK-4000, Denmark. FAU - Christensen, Peter AU - Christensen P FAU - Tuchsen, Erik AU - Tuchsen E FAU - Lund, Torben AU - Lund T LA - eng PT - Journal Article PT - Validation Study DEP - 20031125 PL - England TA - Analyst JT - The Analyst JID - 0372652 RN - 0 (Coenzymes) RN - 1339-63-5 (Ubiquinone) RN - EJ27X76M46 (coenzyme Q10) SB - IM MH - Chromatography, Liquid/methods MH - Coenzymes MH - Humans MH - Mass Spectrometry/methods MH - Oxidation-Reduction MH - Ubiquinone/*analogs & derivatives/*blood EDAT- 2004/01/23 05:00 MHDA- 2004/04/17 05:00 CRDT- 2004/01/23 05:00 PHST- 2004/01/23 05:00 [pubmed] PHST- 2004/04/17 05:00 [medline] PHST- 2004/01/23 05:00 [entrez] AID - 10.1039/b308690a [doi] PST - ppublish SO - Analyst. 2004 Jan;129(1):45-50. doi: 10.1039/b308690a. Epub 2003 Nov 25.