PMID- 14738135
OWN - NLM
STAT- MEDLINE
DCOM- 20040302
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 44
IP  - 11
DP  - 2003 Nov
TI  - Multicolor karyotyping in acute myeloid leukemia.
PG  - 1843-53
AB  - Cytogenetic data have significantly contributed to our understanding of the 
      heterogeneity of acute myeloid leukemia (AML). In AML, numerous recurrent 
      chromosomal aberrations have been identified, and several of them, e.g. 
      t(8;21)(q22;q22), t(15;17)(q22;q11-12), inv(16)(p13q22), are specific for 
      distinct subgroups. Furthermore, chromosomal aberrations have proved to be of 
      paramount prognostic importance for remission induction and survival. Chromosome 
      analysis using classical cytogenetic banding techniques often fails to completely 
      resolve complex karyotypes and cryptic translocations not identifiable by these 
      techniques have been detected using molecular cytogenetic methods. While 
      fluorescence in situ hybridization (FISH) has become an indispensable tool for 
      screening and follow-up of known aberrations, the techniques of spectral 
      karyotyping (SKY) and multiplex-fluorescence in situ hybridization (M-FISH) allow 
      for the simultaneous visualization of all chromosomes of a metaphase in a single 
      hybridization step, and thereby enable screening for the aberrations present 
      without their prior knowledge. Therefore, with the introduction of these 
      techniques in 1996 the comprehensive analysis of complex karyotypes and the 
      identification of new, hitherto cryptic translocations and, ultimately, the 
      identification of new disease subgroups seemed possible. Since, more than 600 
      cases of AML and MDS have been analyzed. Herein, we attempt to summarize the data 
      published and discuss what has been achieved towards realization of these goals.
FAU - Tchinda, Joelle
AU  - Tchinda J
AD  - Institut fur Humangenetik, Universitatsklinikum Munster, Vesaliusweg 12-14, 48129 
      Munster, Germany. tchinda@uni.muenster.de
FAU - Volpert, Sarah
AU  - Volpert S
FAU - McNeil, Nicole
AU  - McNeil N
FAU - Neumann, Thomas
AU  - Neumann T
FAU - Kennerknecht, Ingo
AU  - Kennerknecht I
FAU - Ried, Thomas
AU  - Ried T
FAU - Buchner, Thomas
AU  - Buchner T
FAU - Serve, Hubert
AU  - Serve H
FAU - Berdel, Wolfgang E
AU  - Berdel WE
FAU - Horst, Jurgen
AU  - Horst J
FAU - Hilgenfeld, Eva
AU  - Hilgenfeld E
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Leukemia, Myeloid/*genetics
MH  - Translocation, Genetic
RF  - 68
EDAT- 2004/01/24 05:00
MHDA- 2004/03/03 05:00
CRDT- 2004/01/24 05:00
PHST- 2004/01/24 05:00 [pubmed]
PHST- 2004/03/03 05:00 [medline]
PHST- 2004/01/24 05:00 [entrez]
AID - 10.1080/10428190310001603605 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2003 Nov;44(11):1843-53. doi: 10.1080/10428190310001603605.