PMID- 14738910
OWN - NLM
STAT- MEDLINE
DCOM- 20040309
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 74
IP  - 13
DP  - 2004 Feb 13
TI  - Interleukin-18 induces serum amyloid A (SAA) protein production from rheumatoid 
      synovial fibroblasts.
PG  - 1671-9
AB  - Interleukin-18 (IL-18) is a novel proinflammatory cytokine that was recently 
      found in synovial fluids and synovial tissues from patients with rheumatoid 
      arthritis (RA). To investigate the role of IL-18 in rheumatoid synovitis, the 
      levels of IL-18 and serum amyloid A (SAA) were measured in synovial fluids from 
      24 patients with rheumatoid arthritis (RA) and 13 patients with osteoarthritis 
      (OA). The levels of IL-18 and SAA in the synovial fluids were elevated in RA 
      patients. In contrast, the levels of IL-18 in synovial fluids from OA patients 
      were significantly lower compared to those of RA patients. SAA was not detected 
      in synovial fluids from OA patients. The expression of SAA mRNA in rheumatoid 
      synovial cells was also examined. SAA4 mRNA, which was constitutively expressed 
      by rheumatoid synovial cells, was not affected by IL-18 stimulation. Although 
      acute phase SAA (A-SAA, SAA1 + 2) mRNA was not detected in unstimulated synovial 
      cells, its expression was induced by IL-18 stimulation. By immunoblot, we 
      demonstrated that IL-18 induced the SAA protein synthesis from rheumatoid 
      synovial cells in a dose-dependent manner. These results indicate a novel role 
      for IL-18 in rheumatoid inflammation through the synovial SAA production.
FAU - Tanaka, Fumiko
AU  - Tanaka F
AD  - First Department of Internal Medicine, Nagasaki University School of Medicine, 
      Sakamoto 1-7-1, Nagasaki, Japan.
FAU - Migita, Kiyoshi
AU  - Migita K
FAU - Kawabe, Yojiro
AU  - Kawabe Y
FAU - Aoyagi, Takahiko
AU  - Aoyagi T
FAU - Ida, Hiroaki
AU  - Ida H
FAU - Kawakami, Atsushi
AU  - Kawakami A
FAU - Eguchi, Katsumi
AU  - Eguchi K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (IL18R1 protein, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
RN  - 0 (Serum Amyloid A Protein)
SB  - IM
MH  - Arthritis, Rheumatoid/immunology/*metabolism/pathology
MH  - Cells, Cultured
MH  - Fibroblasts/cytology/immunology/*metabolism
MH  - Humans
MH  - Interleukin-18/immunology/*metabolism
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Receptors, Interleukin/genetics/metabolism
MH  - Receptors, Interleukin-18
MH  - Serum Amyloid A Protein/immunology/*metabolism
MH  - Statistics as Topic
MH  - Synovial Fluid/chemistry
MH  - Synovial Membrane/*cytology/pathology
EDAT- 2004/01/24 05:00
MHDA- 2004/03/10 05:00
CRDT- 2004/01/24 05:00
PHST- 2004/01/24 05:00 [pubmed]
PHST- 2004/03/10 05:00 [medline]
PHST- 2004/01/24 05:00 [entrez]
AID - S0024320503010580 [pii]
AID - 10.1016/j.lfs.2003.08.025 [doi]
PST - ppublish
SO  - Life Sci. 2004 Feb 13;74(13):1671-9. doi: 10.1016/j.lfs.2003.08.025.