PMID- 14741757
OWN - NLM
STAT- MEDLINE
DCOM- 20040503
LR  - 20220409
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 147
IP  - 1-2
DP  - 2003 Dec 30
TI  - Altered neuron-glia interactions in a low, chronic prenatal ethanol exposure.
PG  - 119-33
AB  - Serotoninergic neurons, astrocytes and nitrergic system play an important role in 
      central nervous system (CNS) development. These systems are altered in prenatal 
      ethanol exposure (PEE) but ethanol (EtOH) effects may be very diverse under 
      different conditions. In this study, we analyzed morphologically two 
      serotoninergic mesencephalic nuclei and three prosencephalic areas of 
      serotoninergic innervation in a model of pre- and postnatal low-ethanol exposure. 
      Female Wistar rats were orally exposed to EtOH 6.6% (v/v), ad libitum, for 6 
      weeks before mating and during gestation and lactation while control group 
      received water ad libitum. Twenty-day-old offspring (P21) brains were processed 
      and immunoreactivity (IR) using antibodies against tryptophan hydroxylase (TPH), 
      5-HT, 5-HT transporter (5HTT), glial fibrillary acidic protein (GFAP), S-100B 
      protein, 200-kDa neurofilaments (Nf-200) and neuronal nitric oxide synthase 
      (nNOS) was evaluated. Dorsal and median raphe nucleus (DRN and MRN), hippocampus 
      (Hipp), striatum (Strt) and frontal cortex (FCx) were studied by 
      computer-assisted image analysis. Relative optical density (ROD) of TPH-IR, 
      5-HT-IR and nNOS-IR neurons; cell area of GFAP-IR astrocytes; relative area of 
      5HTT-IR fibers and Nf-200-IR were evaluated. TPH-IR was increased in DRN and MRN 
      and 5-HT-IR was increased only in MRN. 5-HTT-IR fibers and ROD of S-100B-IR 
      astrocytes were increased in the three prosencephalic areas while GFAP-IR 
      astrocytes were hypertrophied only in Hipp and FCx. Nf-200 expression was 
      increased in Hipp and Strt and morphologically altered in the FCx. ROD of nNOS-IR 
      neurons was increased in Strt and FCx but was not detected in Hipp. We have also 
      detected morphological changes resembling accelerated development and maturation, 
      and early aging. Considering the evidences of a close 5-HT-astroglial-NO 
      relationship during CNS development the differential response of the studied 
      regions is an interesting result that could be due to different gradients of 
      development in the studied areas and/or different responses of those areas to the 
      effects of a low pre- and postnatal ethanol exposure.
FAU - Evrard, Sergio Gustavo
AU  - Evrard SG
AD  - Instituto de Biologi;a Celular y Neurociencias Prof. Eduardo de Robertis, 
      Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, (C1121ABG), 
      Buenos Aires, Argentina.
FAU - Vega, Maite Duhalde
AU  - Vega MD
FAU - Ramos, Alberto Javier
AU  - Ramos AJ
FAU - Tagliaferro, Patricia
AU  - Tagliaferro P
FAU - Brusco, Alicia
AU  - Brusco A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (S100 Calcium Binding Protein beta Subunit)
RN  - 0 (S100 Proteins)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 333DO1RDJY (Serotonin)
RN  - 3K9958V90M (Ethanol)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nos1 protein, rat)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
SB  - IM
MH  - Animals
MH  - Brain Chemistry/drug effects
MH  - Ethanol/*toxicity
MH  - Female
MH  - Frontal Lobe/cytology/drug effects/metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Hippocampus/cytology/drug effects/metabolism
MH  - Immunohistochemistry
MH  - Neostriatum/cytology/drug effects/metabolism
MH  - Nerve Growth Factors
MH  - Neuroglia/*drug effects/metabolism
MH  - Neuronal Plasticity/drug effects
MH  - Neurons/*drug effects/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase/biosynthesis
MH  - Nitric Oxide Synthase Type I
MH  - Phenotype
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Raphe Nuclei/cytology/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - S100 Calcium Binding Protein beta Subunit
MH  - S100 Proteins/metabolism
MH  - Serotonin/metabolism
MH  - Tissue Fixation
MH  - Tryptophan Hydroxylase/metabolism
EDAT- 2004/01/27 05:00
MHDA- 2004/05/05 05:00
CRDT- 2004/01/27 05:00
PHST- 2004/01/27 05:00 [pubmed]
PHST- 2004/05/05 05:00 [medline]
PHST- 2004/01/27 05:00 [entrez]
AID - S0165380603002748 [pii]
AID - 10.1016/j.devbrainres.2003.09.004 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 2003 Dec 30;147(1-2):119-33. doi: 
      10.1016/j.devbrainres.2003.09.004.