PMID- 14742524
OWN - NLM
STAT- MEDLINE
DCOM- 20040218
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 72
IP  - 2
DP  - 2004 Feb
TI  - Immunoglobulin E and eosinophil-dependent protective immunity to larval 
      Onchocerca volvulus in mice immunized with irradiated larvae.
PG  - 810-7
AB  - Mice immunized with irradiated Onchocerca volvulus third-stage larvae developed 
      protective immunity. Eosinophil levels were elevated in the parasite 
      microenvironment at the time of larval killing, and measurements of total serum 
      antibody levels revealed an increase in the immunoglobulin E (IgE) level in 
      immunized mice. The goal of the present study was to identify the role of 
      granulocytes and antibodies in the protective immune response to the larval 
      stages of O. volvulus in mice immunized with irradiated larvae. Immunity did not 
      develop in mice if granulocytes, including both neutrophils and eosinophils, were 
      eliminated, nor did it develop if only eosinophils were eliminated. Moreover, 
      larvae were killed in naive interleukin-5 transgenic mice, and the killing 
      coincided with an increase in the number of eosinophils and the eosinophil 
      peroxidase (EPO) level in the animals. To determine if EPO was required for 
      protective immunity, mice that were genetically deficient in EPO were immunized, 
      and there were no differences in the rates of parasite recovery in EPO-deficient 
      mice and wild-type mice. Two mouse strains were used to study B-cell function; 
      micro MT mice lacked all mature B cells, and Xid mice had deficiencies in the B-1 
      cell population. Immunity did not develop in the micro MT mice but did develop in 
      the Xid mice. Finally, protective immunity was abolished in mice treated to 
      eliminate IgE from the blood. We therefore concluded that IgE and eosinophils are 
      required for adaptive protective immunity to larval O. volvulus in mice.
FAU - Abraham, David
AU  - Abraham D
AD  - Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson 
      University, Philadelphia, Pennsylvania 19107, USA. David.Abraham@jefferson.edu
FAU - Leon, Ofra
AU  - Leon O
FAU - Schnyder-Candrian, Silvia
AU  - Schnyder-Candrian S
FAU - Wang, Chun Chi
AU  - Wang CC
FAU - Galioto, Ann Marie
AU  - Galioto AM
FAU - Kerepesi, Laura A
AU  - Kerepesi LA
FAU - Lee, James J
AU  - Lee JJ
FAU - Lustigman, Sara
AU  - Lustigman S
LA  - eng
GR  - R01 AI047189/AI/NIAID NIH HHS/United States
GR  - AI 42328/AI/NIAID NIH HHS/United States
GR  - AI 47189/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Interleukin-5)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/therapeutic use
MH  - B-Lymphocytes/immunology
MH  - Eosinophils/*immunology
MH  - Granulocytes/immunology
MH  - Immunization
MH  - Immunoglobulin E/*immunology
MH  - Interleukin-5/physiology
MH  - Larva/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Onchocerca volvulus/*immunology
PMC - PMC321619
EDAT- 2004/01/27 05:00
MHDA- 2004/02/19 05:00
PMCR- 2004/02/01
CRDT- 2004/01/27 05:00
PHST- 2004/01/27 05:00 [pubmed]
PHST- 2004/02/19 05:00 [medline]
PHST- 2004/01/27 05:00 [entrez]
PHST- 2004/02/01 00:00 [pmc-release]
AID - 1102 [pii]
AID - 10.1128/IAI.72.2.810-817.2004 [doi]
PST - ppublish
SO  - Infect Immun. 2004 Feb;72(2):810-7. doi: 10.1128/IAI.72.2.810-817.2004.