PMID- 14742992
OWN - NLM
STAT- MEDLINE
DCOM- 20040227
LR  - 20181130
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 77
IP  - 2
DP  - 2004 Jan 27
TI  - Allogeneic reaction induces dendritic cell maturation through proinflammatory 
      cytokine secretion.
PG  - 267-75
AB  - BACKGROUND: A bone marrow transplantation conditioning regimen is known to 
      activate host dendritic cells (DC), which then become able to initiate 
      graft-versus-host disease (GVHD) by presenting alloantigens. In this article, the 
      authors addressed whether the alloreaction could reciprocally maintain DC in an 
      activation state through secretion of proinflammatory cytokines. METHODS: Skin 
      biopsy specimens from GVHD patients were analyzed for the presence of DC. 
      Supernatants collected from primary major histocompatibility antigen (allogeneic 
      mixed leukocyte reaction [MLR] supernatant [SN]) or secondary minor 
      histocompatibility antigen-mismatched mixed lymphocyte reactions were used to 
      culture cytokine-promoted immature (im) DC. DC phenotype, function, and migration 
      were analyzed. RESULTS: Immunostaining from GVHD skin biopsy specimens showed a 
      deficit of Langerhans cells (LC) in the epidermis but the presence of mature DC 
      in the dermis. Because LC should have recovered in the epidermis by this time, 
      the authors then addressed whether the allogeneic reaction could maintain DC in 
      an activation and migratory state, through secretion of inflammatory cytokines. 
      With this aim, cytokine-mediated imDC were exposed to alloMLR-SN for 2 days. The 
      authors observed that DC increased their expression of CD80, CD86, CD40, and 
      human leukocyte antigen (HLA)-DR and neoexpressed CD83, DC-LAMP/CD208, and CCR7. 
      At the functional level, alloMLR-SN-treated DC lost their ability to capture 
      dextran, improved their allostimulatory capacity, and migrated in response to 
      macrophage inflammatory protein 3beta. Interestingly, SN collected from secondary 
      HLA-identical but minor histocompatibility antigen-mismatched MLR induced almost 
      equivalent DC phenotypic maturation. CONCLUSIONS: The authors' results show that 
      the allogeneic reaction leads to maturation and migration of DC through 
      proinflammatory cytokine secretion. This might contribute to the impairment of LC 
      reconstitution in the skin of patients with GVHD.
FAU - Laurin, David
AU  - Laurin D
AD  - Cell Therapy and Immunology Department, Etablissement Francais du Sang 
      Rhone-Alpes, Lyon, France.
FAU - Kanitakis, Jean
AU  - Kanitakis J
FAU - Bienvenu, Jacques
AU  - Bienvenu J
FAU - Bardin, Christine
AU  - Bardin C
FAU - Bernaud, Jeanine
AU  - Bernaud J
FAU - Lebecque, Serge
AU  - Lebecque S
FAU - Gebuhrer, Lucette
AU  - Gebuhrer L
FAU - Rigal, Dominique
AU  - Rigal D
FAU - Eljaafari, Assia
AU  - Eljaafari A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Surface)
RN  - 0 (CD207 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interleukins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, CD
MH  - Antigens, Surface/analysis
MH  - Biopsy
MH  - Blood Donors
MH  - Cell Differentiation/drug effects/immunology
MH  - Cytokines/*metabolism/*pharmacology
MH  - Dendritic Cells/drug effects/*physiology
MH  - Graft vs Host Disease/*immunology/pathology
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Inflammation/physiopathology
MH  - Interferon-gamma/metabolism
MH  - Interleukins/metabolism
MH  - Lectins, C-Type/analysis
MH  - Lymphocyte Culture Test, Mixed
MH  - Mannose-Binding Lectins/analysis
MH  - Monocytes/cytology/drug effects/*immunology
MH  - Reference Values
MH  - Skin/immunology/pathology
MH  - Transplantation, Homologous/*immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2004/01/27 05:00
MHDA- 2004/02/28 05:00
CRDT- 2004/01/27 05:00
PHST- 2004/01/27 05:00 [pubmed]
PHST- 2004/02/28 05:00 [medline]
PHST- 2004/01/27 05:00 [entrez]
AID - 10.1097/01.TP.0000101006.39475.41 [doi]
PST - ppublish
SO  - Transplantation. 2004 Jan 27;77(2):267-75. doi: 
      10.1097/01.TP.0000101006.39475.41.