PMID- 14743431
OWN - NLM
STAT- MEDLINE
DCOM- 20040412
LR  - 20071115
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 75
IP  - 4
DP  - 2004 Feb 15
TI  - Differential subcellular distribution of Ca2+/calmodulin-dependent protein kinase 
      II isoforms in the striatum and NG108-15 cells.
PG  - 480-90
AB  - Four subunits of Ca2+/calmodulin-dependent protein kinase II (CaM KII) have 
      several isoforms, which differ in the variable domain. We previously reported 
      that all subunits were highly expressed in rat striatal neurons. To examine 
      intracellular distributions of CaM KII subunits in the rat striatal neurons, we 
      performed immunoblot analysis with antibodies specific to each subunit in cell 
      extracts from the rat striatum after continuous sucrose density gradient 
      fractionation. The alpha subunit, but not the beta, gamma, or delta subunits, was 
      colocalized with synapsin I, and each subunit showed a distinct distribution 
      pattern in the fractions. To examine further the intracellular distributions of 
      CaM KII isoforms in the same subunit, we established NG108-15 cells stably 
      expressing delta1, delta3, and delta4 isoforms and examined distributions of the 
      delta and gamma isoforms in these cell lines after fractionation. Each of the 
      overexpressed exogenous delta isoforms showed a distinct distribution pattern. 
      The endogenous delta2 was colocalized with the overexpressed delta1, delta3, and 
      delta4 isoforms. However, the endogenous gammaB/gammaC isoforms were not 
      colocalized with the overexpressed delta isoforms. Furthermore, the endogenous 
      delta1 was concentrated in the microsomal fraction from the rat striatum. With 
      the results taken together, it is suggested that CaM KII forms oligomers between 
      isoforms in the same subunit but not in different subunits. The variable domain 
      of CaM KII isoforms might possibly be responsible for targeting to certain 
      intracellular compartments.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Takeuchi, Yusuke
AU  - Takeuchi Y
AD  - Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku 
      University, Aramaki-Aoba-ku, Sendai 980-8578, Japan. 
      yusuket@mail.pharm.tohoku.ac.jp
FAU - Nomura, Kazumi
AU  - Nomura K
FAU - Fukunaga, Kohji
AU  - Fukunaga K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Isoenzymes)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/genetics/*metabolism
MH  - Cell Line
MH  - Centrifugation, Density Gradient
MH  - Corpus Striatum/cytology/*enzymology
MH  - Isoenzymes/genetics/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Rats
MH  - Subcellular Fractions/enzymology
EDAT- 2004/01/27 05:00
MHDA- 2004/04/13 05:00
CRDT- 2004/01/27 05:00
PHST- 2004/01/27 05:00 [pubmed]
PHST- 2004/04/13 05:00 [medline]
PHST- 2004/01/27 05:00 [entrez]
AID - 10.1002/jnr.20010 [doi]
PST - ppublish
SO  - J Neurosci Res. 2004 Feb 15;75(4):480-90. doi: 10.1002/jnr.20010.