PMID- 14744132
OWN - NLM
STAT- MEDLINE
DCOM- 20040520
LR  - 20200401
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 43
IP  - 4
DP  - 2004 Feb 3
TI  - Solution structure of a CCHHC domain of neural zinc finger factor-1 and its 
      implications for DNA binding.
PG  - 898-903
AB  - The structure of a CCHHC zinc-binding domain from neural zinc finger factor-1 
      (NZF-1) has been determined in solution though the use of NMR methods. This 
      domain is a member of a family of domains that have the 
      Cys-X(4)-Cys-X(4)-His-X(7)-His-X(5)-Cys consensus sequence. The structure 
      determination reveals a novel fold based around a zinc(II) ion coordinated to 
      three Cys residues and the second of the two conserved His residues. The other 
      His residue is stacked between the metal-coordinated His residue and a relatively 
      conserved aromatic residue. Analysis of His to Gln sequence variants reveals that 
      both His residues are required for the formation of a well-defined structure, but 
      neither is required for high-affinity metal binding at a tetrahedral site. The 
      structure suggests that a two-domain protein fragment and a double-stranded DNA 
      binding site may interact with a common two-fold axis relating the two domains 
      and the two half-sites of the DNA-inverted repeat.
FAU - Berkovits-Cymet, Holly J
AU  - Berkovits-Cymet HJ
AD  - Department of Biophysics and Biophysical Chemistry, Johns Hopkins University 
      School of Medicine, Baltimore, Maryland 21205, USA.
FAU - Amann, Barbara T
AU  - Amann BT
FAU - Berg, Jeremy M
AU  - Berg JM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Myt1l protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Trans-Activators)
RN  - 4QD397987E (Histidine)
RN  - 9007-49-2 (DNA)
RN  - J41CSQ7QDS (Zinc)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Amino Acid Motifs/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites/genetics
MH  - Cysteine/chemistry
MH  - DNA/*chemistry/genetics/*metabolism
MH  - DNA Mutational Analysis
MH  - Histidine/chemistry
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*chemistry/genetics/*metabolism
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Peptide Fragments/chemistry/genetics/metabolism
MH  - Protein Binding/genetics
MH  - Protein Structure, Tertiary/genetics
MH  - Rats
MH  - Thermodynamics
MH  - Trans-Activators/*chemistry/genetics/*metabolism
MH  - Zinc/chemistry
MH  - *Zinc Fingers/genetics
EDAT- 2004/01/28 05:00
MHDA- 2004/05/21 05:00
CRDT- 2004/01/28 05:00
PHST- 2004/01/28 05:00 [pubmed]
PHST- 2004/05/21 05:00 [medline]
PHST- 2004/01/28 05:00 [entrez]
AID - 10.1021/bi035159d [doi]
PST - ppublish
SO  - Biochemistry. 2004 Feb 3;43(4):898-903. doi: 10.1021/bi035159d.