PMID- 14745850
OWN - NLM
STAT- MEDLINE
DCOM- 20040920
LR  - 20131121
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 24
IP  - 1
DP  - 2004 Jan-Feb
TI  - Assessment of the skin sensitization potency of eugenol and its dimers using a 
      non-radioisotopic modification of the local lymph node assay.
PG  - 77-81
AB  - Allergic contact dermatitis is a serious health problem. There is a need to 
      identify and characterize skin sensitization hazards, particularly with respect 
      to relative potency, so that accurate risk assessments can be developed. For 
      these purposes the murine local lymph node assay (LLNA) was developed. Here, we 
      have investigated further a modi fi cation of this assay, non-radioisotopic LLNA, 
      which in place of tritiated thymidine to measure lymph node cell proliferation 
      employs incorporation of 5-bromo-2'-deoxyuridine. Using this method we have 
      examined the skin sensitizing activity of eugenol, a known human contact 
      allergen, and its dimers 2,2'-dihydroxyl-3,3'-dimethoxy-5,5'-diallyl-biphenyl 
      (DHEA) and 4,5'-diallyl-2'-hydroxy-2,3'-dimethoxy phenyl ether (DHEB). Activity 
      in the guinea pig maximization test (GPMT) also measured. On the basis of GPMT 
      assays, eugenol was classified as a mild skin sensitizer, DHEA as a weak skin 
      sensitizer and DHEB as an extreme skin sensitizer. In the non-radioisotopic LLNA 
      all chemicals were found to give positive responses insofar as each was able to 
      provoke a stimulation index (SI) of >or=3 at one or more test concentrations. The 
      relative skin sensitizing potency of these chemicals was evaluated in the 
      non-radioisotopic LLNA by derivation of an ec(3) value (the concentration of 
      chemical required to provoke an SI of 3). The ec(3) values calculated were 25.1% 
      for eugenol, >30% for DHEA and 2.3% for DHEB. Collectively these data suggest 
      that assessments of relative potency deriving from non-radioisotopic LLNA 
      responses correlate well with evaluations based on GPMT results. These 
      investigations provide support for the proposal that the non-radioisotopic LLNA 
      may serve as an effective alternative to the GPMT where there is a need to avoid 
      the use of radioisotopes.
CI  - Copyright 2004 John Wiley & Sons, Ltd.
FAU - Takeyoshi, Masahiro
AU  - Takeyoshi M
AD  - Chemicals Assessment Center, Chemicals Evaluation and Research Institute, 3-822, 
      Ishii-machi, Hita-shi, Oita 8770061, Japan. takeyoshi-masahiro@ceri.jp
FAU - Noda, Shuji
AU  - Noda S
FAU - Yamazaki, Shunsuke
AU  - Yamazaki S
FAU - Kakishima, Hiroshi
AU  - Kakishima H
FAU - Yamasaki, Kanji
AU  - Yamasaki K
FAU - Kimber, Ian
AU  - Kimber I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (2,2'-dihydroxy-3,3'-dimethoxy-5,5'-diallylbiphenyl)
RN  - 0 (4,5'-diallyl-1,2'-dihydroxy-2,3'-dimethoxyphenyl ether)
RN  - 0 (Allergens)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Phenyl Ethers)
RN  - 3T8H1794QW (Eugenol)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - Biphenyl Compounds/*toxicity
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Division/drug effects/physiology
MH  - Dermatitis, Allergic Contact/etiology/metabolism/pathology
MH  - Dimerization
MH  - Dose-Response Relationship, Immunologic
MH  - Eugenol/analogs & derivatives/*toxicity
MH  - Female
MH  - Guinea Pigs
MH  - Local Lymph Node Assay
MH  - Lymph Nodes/drug effects/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Phenyl Ethers/*toxicity
MH  - Reproducibility of Results
MH  - Skin/*drug effects/immunology
EDAT- 2004/01/28 05:00
MHDA- 2004/09/21 05:00
CRDT- 2004/01/28 05:00
PHST- 2004/01/28 05:00 [pubmed]
PHST- 2004/09/21 05:00 [medline]
PHST- 2004/01/28 05:00 [entrez]
AID - 10.1002/jat.951 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2004 Jan-Feb;24(1):77-81. doi: 10.1002/jat.951.