PMID- 14751295
OWN - NLM
STAT- MEDLINE
DCOM- 20040412
LR  - 20190712
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 123
IP  - 4
DP  - 2004
TI  - Evidence of neuronal excitatory amino acid carrier 1 expression in rat dorsal 
      root ganglion neurons and their central terminals.
PG  - 1045-51
AB  - The expression and distribution of the neuronal glutamate transporter, excitatory 
      amino acid carrier-1 (EAAC1), are demonstrated in the dorsal root ganglion 
      neurons and their central terminals. Reverse transcriptase-polymerase chain 
      reaction shows expression of EAAC1 mRNA in the dorsal root ganglion. 
      Immunoblotting analysis further confirms existence of EAAC1 protein in this 
      region. Immunocytochemistry reveals that approximately 46.6% of the dorsal root 
      ganglion neurons are EAAC1-positive. Most EAAC1-positive neurons are small and 
      around 250-750 microm2 in surface area, and some co-label with calcitonin 
      gene-related peptide (CGRP) or isolectin IB4. In the spinal cord, EAAC-1 
      immunoreactive small dot- or patch-like structures are mainly localized in the 
      superficial dorsal horn, and some are positive for CGRP or labeled by isolectin 
      IB4. Unilateral dorsal rhizotomy experiments further show that EAAC1 
      immunoreactivity is less intense in superficial dorsal horn on the side 
      ipsilateral to the dorsal rhizotomy than on the contralateral side. The results 
      indicate the presence of EAAC1 in the dorsal root ganglion neurons and their 
      central terminals. Our findings suggest that EAAC1 might play an important role 
      in transmission and modulation of nociceptive information via the regulation of 
      pre-synaptically released glutamate.
FAU - Tao, F
AU  - Tao F
AD  - Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University 
      School of Medicine, 355 Ross, 720 Rutland Avenue, Baltimore, MD 21205, USA. 
      ytau@jhmi.edu
FAU - Liaw, W-J
AU  - Liaw WJ
FAU - Zhang, B
AU  - Zhang B
FAU - Yaster, M
AU  - Yaster M
FAU - Rothstein, J D
AU  - Rothstein JD
FAU - Johns, R A
AU  - Johns RA
FAU - Tao, Y-X
AU  - Tao YX
LA  - eng
GR  - NS44219/NS/NINDS NIH HHS/United States
GR  - R01 GM49111/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Excitatory Amino Acid Transporter 3)
RN  - 0 (Glutamate Plasma Membrane Transport Proteins)
RN  - 0 (Lectins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Slc1a1 protein, rat)
RN  - 0 (Symporters)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Amino Acid Transport System X-AG/genetics/*metabolism
MH  - Animals
MH  - Blotting, Western/methods
MH  - Calcitonin Gene-Related Peptide/metabolism
MH  - Cell Count
MH  - Excitatory Amino Acid Transporter 3
MH  - Functional Laterality
MH  - Ganglia, Spinal/*cytology
MH  - Glutamate Plasma Membrane Transport Proteins
MH  - Immunohistochemistry/methods
MH  - Lectins/metabolism
MH  - Male
MH  - Neurons/*metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Rhizotomy/methods
MH  - Spinal Cord/metabolism
MH  - Symporters/genetics/*metabolism
EDAT- 2004/01/31 05:00
MHDA- 2004/04/13 05:00
CRDT- 2004/01/31 05:00
PHST- 2004/01/31 05:00 [pubmed]
PHST- 2004/04/13 05:00 [medline]
PHST- 2004/01/31 05:00 [entrez]
AID - S0306452203008674 [pii]
AID - 10.1016/j.neuroscience.2003.11.026 [doi]
PST - ppublish
SO  - Neuroscience. 2004;123(4):1045-51. doi: 10.1016/j.neuroscience.2003.11.026.