PMID- 14751798
OWN - NLM
STAT- MEDLINE
DCOM- 20041005
LR  - 20191210
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 801
IP  - 2
DP  - 2004 Mar 5
TI  - Rapid screening procedure based on headspace solid-phase microextraction and gas 
      chromatography-mass spectrometry for the detection of many recreational drugs in 
      hair.
PG  - 289-96
AB  - An increasing number of synthetic drugs are appearing on the illicit market and 
      on the scene of drug use by youngsters. Official figures are underestimated. In 
      addition, immunochemical tests are blind to many of these drugs and appropriate 
      analytical procedures for routine clinical and epidemiological purposes are 
      lacking. Therefore, the perceived increasing abuse of recreational drugs has not 
      been proved yet. In a previous paper, we proposed a procedure for the preliminary 
      screening of several recreational substances in hair and other biological 
      matrices. Unfortunately, this procedure cannot apply to cocaine. Consequently, we 
      performed a new headspace solid-phase microextraction and gas chromatography-mass 
      spectrometry (HS-SPME-GC-MS) procedure for the simultaneous detection of cocaine, 
      amphetamine (A), methamphetamine (MA), methylen-dioxyamphetamine (MDA), 
      methylen-dioxymethamphetamine (MDMA), methylen-dioxyethamphetamine (MDE), 
      N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine (MBDB), ketamine, and methadone in 
      human hair. Hair was washed with water and acetone in an ultrasonic bath. A short 
      acid extraction with 1M hydrochloric acid was needed; the fiber was exposed to a 
      5 min absorption at 90 degrees C and thermal desorption was performed at 250 
      degrees C for 3 min. The procedure was simple, rapid, required small quantities 
      of sample and no derivatization. Good linearity was obtained over the 0.1-20.0 
      ng/mg range for the target compounds. Sensitivity was good enough: limits of 
      detection (LOD) were 0.7 ng/mg of hair for the majority of substances. The 
      intra-day precision ranged between 7 and 20%. This paper deals with the 
      analytical performance of this procedure and its preliminary application to hair 
      samples obtained on a voluntary basis from 183 young people (138 males and 45 
      females) in the Rome area.
FAU - Gentili, Stefano
AU  - Gentili S
AD  - Clinical Biochemistry Department, Drug Abuse Section, Istituto Superiore di 
      Sanita, Viale Regina Elena 299, 00161 Rome, Italy.
FAU - Cornetta, Maria
AU  - Cornetta M
FAU - Macchia, Teodora
AU  - Macchia T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Amphetamines)
RN  - 0 (Illicit Drugs)
RN  - I5Y540LHVR (Cocaine)
RN  - QTT17582CB (Hydrochloric Acid)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Amphetamines/analysis
MH  - Cocaine/analysis
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Hair/*chemistry
MH  - Humans
MH  - Hydrochloric Acid
MH  - Illicit Drugs/*analysis
MH  - Male
MH  - Quality Control
MH  - Sensitivity and Specificity
EDAT- 2004/01/31 05:00
MHDA- 2004/10/06 09:00
CRDT- 2004/01/31 05:00
PHST- 2004/01/31 05:00 [pubmed]
PHST- 2004/10/06 09:00 [medline]
PHST- 2004/01/31 05:00 [entrez]
AID - S1570023203010079 [pii]
AID - 10.1016/j.jchromb.2003.11.034 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Mar 5;801(2):289-96. doi: 
      10.1016/j.jchromb.2003.11.034.