PMID- 14756503
OWN - NLM
STAT- MEDLINE
DCOM- 20041005
LR  - 20190702
IS  - 0042-4900 (Print)
IS  - 0042-4900 (Linking)
VI  - 154
IP  - 3
DP  - 2004 Jan 17
TI  - Studies on the carriage and transmission of porcine reproductive and respiratory 
      syndrome virus by individual houseflies (Musca domestica).
PG  - 80-5
AB  - The objectives of the study were to determine the site of porcine reproductive 
      and respiratory syndrome virus (PRRSV) in individual houseflies, to assess 
      whether an individual housefly could transmit PRRSV to a susceptible pig, and to 
      compare the ability of PCR, virus isolation and a pig bioassay to detect PRRSV in 
      houseflies. In the first experiment 26 houseflies were fed on a pig infected 
      experimentally with PRRSV; 13 were processed as a whole fly homogenate, while an 
      exterior surface wash and a gut homogenate were collected from the other 13. 
      Infectious PRRSV was recovered from nine of the whole fly homogenates, 12 of the 
      gut homogenates and one of the exterior surface washes. In the second experiment, 
      two of 10 individual houseflies, which had fed on an infected pig, transmitted 
      PRRSV to a susceptible pig in a controlled manual transmission protocol. In the 
      third experiment, single flies or pools of 30 flies were immersed in different 
      concentrations of a PRRSV inoculum, then tested by PCR, virus isolation and 
      bioassay. The virus was detected at a concentration of 10(1) TCID50/ml by PCR, 
      10(2) TCID50/ml by the bioassay and 10(3) TCID50/ml by virus isolation.
FAU - Otake, S
AU  - Otake S
AD  - Swine Disease Eradication Center, University of Minnesota College of Veterinary 
      Medicine, 385 Animal Science/ Veterinary Medicine Building, 1988 Fitch Avenue, St 
      Paul, MN 55108, USA.
FAU - Dee, S A
AU  - Dee SA
FAU - Moon, R D
AU  - Moon RD
FAU - Rossow, K D
AU  - Rossow KD
FAU - Trincado, C
AU  - Trincado C
FAU - Pijoan, C
AU  - Pijoan C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Carrier State/veterinary
MH  - DNA, Viral/isolation & purification
MH  - Feeding Behavior
MH  - Houseflies/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/isolation & 
      purification/*pathogenicity
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2004/02/06 05:00
MHDA- 2004/10/06 09:00
CRDT- 2004/02/06 05:00
PHST- 2004/02/06 05:00 [pubmed]
PHST- 2004/10/06 09:00 [medline]
PHST- 2004/02/06 05:00 [entrez]
AID - 10.1136/vr.154.3.80 [doi]
PST - ppublish
SO  - Vet Rec. 2004 Jan 17;154(3):80-5. doi: 10.1136/vr.154.3.80.