PMID- 14756770
OWN - NLM
STAT- MEDLINE
DCOM- 20040607
LR  - 20190906
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 37
IP  - 4
DP  - 2004 Feb
TI  - Forest tent caterpillars (Malacosoma disstria) induce local and systemic diurnal 
      emissions of terpenoid volatiles in hybrid poplar (Populus trichocarpa x 
      deltoides): cDNA cloning, functional characterization, and patterns of gene 
      expression of (-)-germacrene D synthase, PtdTPS1.
PG  - 603-16
AB  - Feeding forest tent caterpillars (FTCs) induced local and systemic diurnal 
      emissions of (-)-germacrene D, along with (E)-beta-ocimene, linalool, 
      (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), benzene cyanide, and 
      (E,E)-alpha-farnesene, from leaves of hybrid poplar. FTC feeding induced 
      substantially higher levels of volatiles in local and systemic leaves than did 
      mechanical wounding. A full-length poplar sesquiterpene synthase cDNA (PtdTPS1) 
      was isolated and functionally identified as (-)-germacrene D synthase. Expression 
      of PtdTPS1, expression of genes of early, intermediate and late steps in 
      terpenoid biosynthesis, and expression of a lipoxygenase gene (PtdLOX1) were 
      analyzed in local FTC-infested and systemic leaves. Transcript levels of PtdTPS1 
      and PtdLOX1 were strongly increased in response to herbivory. PtdTPS1 was also 
      induced by mechanical wounding or by methyl jasmonate (MeJA) treatment. FTC 
      feeding did not affect transcript levels of 3-hydroxy-3-methylglutaryl-CoA 
      reductase (HMGR), 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR), and 
      isoprene synthase (IPS). Two other TPS genes, PtdTPS2 and PtTPS3, and farnesyl 
      diphosphate synthase were only very transiently induced. These results illustrate 
      differential expression of terpenoid pathway genes in response to insect feeding 
      and a key function of (-)-germacrene D synthase PtdTPS1 for herbivore-induced 
      local and systemic volatile emissions in hybrid poplar. FTC-induced transcripts 
      of PtdTPS1 followed diurnal rhythm. Spatial patterns of FTC-induced PtdTPS1 
      transcript accumulation revealed acropetal but not basipetal direction of the 
      systemic response. Implications for tritrophic poplar-FTC-predator/parasitoid 
      interactions are discussed.
FAU - Arimura, Gen-Ichiro
AU  - Arimura G
AD  - Biotechnology Laboratory, University of British Columbia, 6174 University 
      Boulevard, Vancouver, BC, Canada V6T 1Z3.
FAU - Huber, Dezene P W
AU  - Huber DP
FAU - Bohlmann, Jorg
AU  - Bohlmann J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Acetates)
RN  - 0 (Cyclopentanes)
RN  - 0 (DNA, Complementary)
RN  - 0 (Oxylipins)
RN  - 0 (Plant Proteins)
RN  - 0 (Sesquiterpenes, Germacrane)
RN  - 0 (Terpenes)
RN  - 900N171A0F (methyl jasmonate)
RN  - EC 2.5.- (Alkyl and Aryl Transferases)
RN  - EC 2.5.1.- (germacrene D synthase)
RN  - EC 2.5.1.- (isoprene synthase)
RN  - EC 4.1.- (Carbon-Carbon Lyases)
RN  - EC 4.2.3.6 (trichodiene synthetase)
SB  - IM
MH  - Acetates/pharmacology
MH  - Alkyl and Aryl Transferases/*genetics/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carbon-Carbon Lyases/genetics/metabolism
MH  - Cloning, Molecular
MH  - Cyclopentanes/pharmacology
MH  - DNA, Complementary/chemistry/genetics
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Gene Expression Regulation, Plant/drug effects
MH  - Host-Parasite Interactions
MH  - Immunity, Innate/genetics
MH  - Lepidoptera/*growth & development
MH  - Molecular Sequence Data
MH  - Oxylipins
MH  - Phylogeny
MH  - Plant Diseases/genetics/parasitology
MH  - Plant Proteins/*genetics/metabolism
MH  - Populus/chemistry/*genetics/parasitology
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sesquiterpenes, Germacrane/biosynthesis
MH  - Stress, Mechanical
MH  - Terpenes/*metabolism
MH  - Volatilization
EDAT- 2004/02/06 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/02/06 05:00
PHST- 2004/02/06 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/02/06 05:00 [entrez]
AID - 1987 [pii]
AID - 10.1111/j.1365-313x.2003.01987.x [doi]
PST - ppublish
SO  - Plant J. 2004 Feb;37(4):603-16. doi: 10.1111/j.1365-313x.2003.01987.x.