PMID- 14757416
OWN - NLM
STAT- MEDLINE
DCOM- 20040930
LR  - 20191108
IS  - 1079-9796 (Print)
IS  - 1079-9796 (Linking)
VI  - 32
IP  - 1
DP  - 2004 Jan-Feb
TI  - Applications and technical challenges of fluorescence in situ hybridization in 
      stem cell research.
PG  - 68-76
AB  - Stem cell research, maintenance, and manipulations have advanced significantly in 
      recent years, and we now witness successful clinical applications of stem 
      therapies. However, challenges in regard to karyotypic stability and the ploidy 
      status of stem cell lines have been addressed only marginally. Our approach to 
      develop technology to address these highly relevant issues is based on 
      fluorescence in situ hybridization (FISH) using nonisotopically labeled DNA 
      probes. As a single cell analysis technique, FISH is expected to be applicable to 
      a variety of cells and tissues including interphase and metaphase cell 
      preparations as well as tissue sections and biopsy material. Over the last 
      decade, our laboratories generated a large number of probes and probe sets for 
      the molecular cytogenetic analyses of stem cells derived from different species. 
      These probes and the introduction of spectral imaging bring us close to be able 
      to perform a comprehensive karyotype analysis of single interphase cell nuclei. 
      It should furthermore be possible to couple cytogenetic investigations of the 
      cellular genotype with analysis of gene expression. This report summarizes our 
      technical achievements relevant to stem cell research and outlines plans for 
      future research and developments.
FAU - Weier, Heinz-Ulli G
AU  - Weier HU
AD  - Life Sciences Division 74-157, University of California, E.O. Lawrence Berkeley 
      National Laboratory, Berkeley, CA 94720, USA. ugweier@lbl.gov
FAU - Chu, Lisa W
AU  - Chu LW
FAU - Murnane, John P
AU  - Murnane JP
FAU - Weier, Jingly F
AU  - Weier JF
LA  - eng
GR  - CA80792/CA/NCI NIH HHS/United States
GR  - CA88258/CA/NCI NIH HHS/United States
GR  - ES08627/ES/NIEHS NIH HHS/United States
GR  - K25HD041425/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood Cells Mol Dis
JT  - Blood cells, molecules & diseases
JID - 9509932
RN  - 0 (DNA Probes)
SB  - IM
MH  - DNA Probes
MH  - Embryo, Mammalian
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Ploidies
MH  - Spectral Karyotyping
MH  - Stem Cells/*cytology
EDAT- 2004/02/06 05:00
MHDA- 2004/10/01 05:00
CRDT- 2004/02/06 05:00
PHST- 2004/02/06 05:00 [pubmed]
PHST- 2004/10/01 05:00 [medline]
PHST- 2004/02/06 05:00 [entrez]
AID - S1079979603002614 [pii]
AID - 10.1016/j.bcmd.2003.09.017 [doi]
PST - ppublish
SO  - Blood Cells Mol Dis. 2004 Jan-Feb;32(1):68-76. doi: 10.1016/j.bcmd.2003.09.017.