PMID- 14760804
OWN - NLM
STAT- MEDLINE
DCOM- 20040517
LR  - 20151119
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 31
IP  - 2
DP  - 2004 Feb
TI  - Effect of radiosynovectomy with holmium-166 ferric hydroxide macroaggregate on 
      adult equine cartilage.
PG  - 321-8
AB  - OBJECTIVE: To analyze the effect of radiosynovectomy with holmium-166 ferric 
      hydroxide macroaggregate (166Ho-FHMA) on articular cartilage in 6 adult horses. 
      METHODS: Arthritic changes and mechanical properties of articular cartilage were 
      evaluated with arthroscopy and postmortem microscopic analyses. Glycosaminoglycan 
      content was measured by safranin-O staining combined with digital densitometry, 
      uronic acid analyses, and dimethylene blue binding assay. 35S-sulfate labeling 
      and autoradiography were used to localize proteoglycan synthesis and to 
      characterize proteoglycan structures using SDS-agarose gel electrophoresis. 
      Northern hybridizations were performed to measure the mRNA levels for aggrecan 
      and pro-a1(II) collagen in cartilage samples. RESULTS: Histological signs of 
      degeneration were present in the articular cartilage of both control and 
      radiosynovectomized equine joints. Radiosynovectomy did not aggravate 
      degenerative changes or significantly alter the matrix glycosaminoglycan content. 
      A slightly decreased size of proteoglycan monomers was observed 2 months after 
      166Ho-FHMA radiosynovectomy. Tissue analysis of extracted proteoglycans revealed 
      lower 35S incorporation after radiosynovectomy, but corresponding changes could 
      not be observed in aggrecan mRNA levels. Transient downregulation of pro-a1(II) 
      collagen mRNA transcription was observed 5 days after 166Ho-FHMA 
      radiosynovectomy. CONCLUSION: 166Ho-FHMA treatment did not markedly affect the 
      composition or morphology of adult articular cartilage showing mild degeneration. 
      However, minor degradation of proteoglycan monomers and transient downregulation 
      of pro-a1(II) collagen mRNA were observed.
FAU - Makela, Olli T
AU  - Makela OT
AD  - Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
FAU - Lammi, Mikko J
AU  - Lammi MJ
FAU - Uusitalo, Hannele
AU  - Uusitalo H
FAU - Viitanen, Minna
AU  - Viitanen M
FAU - Hyttinen, Mika M
AU  - Hyttinen MM
FAU - Jurvelin, Jukka S
AU  - Jurvelin JS
FAU - Vuorio, Eero
AU  - Vuorio E
FAU - Helminen, Heikki J
AU  - Helminen HJ
FAU - Tulamo, Riitta-Mari
AU  - Tulamo RM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Hydroxides)
RN  - 0 (Sulfates)
RN  - 0 (Sulfur Radioisotopes)
RN  - 0 (holmium-ferric hydroxide)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/metabolism/*pathology
MH  - Female
MH  - Glycosaminoglycans/metabolism
MH  - Horses
MH  - Hydroxides/*therapeutic use
MH  - Sulfates/pharmacokinetics
MH  - Sulfur Radioisotopes
MH  - Synovial Fluid/metabolism
MH  - Synovial Membrane/metabolism/*pathology
MH  - Synovitis/*pathology/*therapy
EDAT- 2004/02/05 05:00
MHDA- 2004/05/18 05:00
CRDT- 2004/02/05 05:00
PHST- 2004/02/05 05:00 [pubmed]
PHST- 2004/05/18 05:00 [medline]
PHST- 2004/02/05 05:00 [entrez]
AID - 0315162X-31-321 [pii]
PST - ppublish
SO  - J Rheumatol. 2004 Feb;31(2):321-8.