PMID- 14761602
OWN - NLM
STAT- MEDLINE
DCOM- 20040429
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 32
IP  - 6
DP  - 2003 Dec
TI  - [Expression of monocyte chemoattractant protein-1 in experimental rat 
      glomerulonephritis is mediated by NF-kappaB/IkappaB signal pathway].
PG  - 548-52
AB  - OBJECTIVE: To investigate the role of NF-kappaB/IkappaB signal pathway in 
      mediating the expression of monocyte chemoattractant protein-1 (MCP-1) in 
      experimental rat glomerulonephritis. METHODS: Nephrotoxic serum nephritis (NTN) 
      was induced by injection of anti-GBM antibody into the tail veins of rats. 
      Electrophoretic mobility shift assay (EMSA) and Western Blot were used to detect 
      the activation of NF-kappaB, nuclear translocation of p65 subunit and degradation 
      of IkappaBalpha and IkappaBbeta in rat renal tissue. MCP-1 expression in 
      glomeruli and renal tubules was also assessed by immunohistochemistry and 
      ribonuclease protection assay. This was further correlated with the activation of 
      NF-kappaB. RESULTS: There was an obvious expression of MCP-1 in glomeruli and 
      renal tubules. Significant up-regulation of NF-kappaB activation, nuclear 
      translocation of p65 subunit, and degradation of IkappaBalpha and IkappaBbeta 
      were also observed in NTN rat renal tissue, as compared to the control group. A 
      positive correlation was noted between NF-kappaB activation and MCP-1 expression. 
      CONCLUSIONS: NF-kappaB/IkappaB signal pathway may play an important pathogenetic 
      role in glomerulonephritis, with mediating the expression of MCP-1.
FAU - Zhang, Ai-hua
AU  - Zhang AH
AD  - Department of Nephrology, Nanjing Children's Hospital, Affiliated to Nanjing 
      Medical University, Nanjing 210008, China. zhaihua@njmu.edu.cn
FAU - Huang, Song-ming
AU  - Huang SM
FAU - Ding, Gui-xia
AU  - Ding GX
FAU - Wu, Yuan-jun
AU  - Wu YJ
FAU - Zhang, Wei-zhen
AU  - Zhang WZ
FAU - Wu, Hong-mei
AU  - Wu HM
FAU - Fei, Li
AU  - Fei L
FAU - Guo, Mei
AU  - Guo M
FAU - Chen, Rong-hua
AU  - Chen RH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Chemokine CCL2)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Glomerulonephritis/chemically induced/genetics/*metabolism
MH  - I-kappa B Proteins/*metabolism
MH  - Kidney Glomerulus/metabolism/pathology
MH  - Kidney Tubules/metabolism/pathology
MH  - Male
MH  - NF-kappa B/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
EDAT- 2004/02/06 05:00
MHDA- 2004/04/30 05:00
CRDT- 2004/02/06 05:00
PHST- 2004/02/06 05:00 [pubmed]
PHST- 2004/04/30 05:00 [medline]
PHST- 2004/02/06 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2003 Dec;32(6):548-52.