PMID- 14761878
OWN - NLM
STAT- MEDLINE
DCOM- 20040722
LR  - 20111117
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 287
IP  - 1
DP  - 2004 Jul
TI  - Regulation of metabolic genes in human skeletal muscle by short-term exercise and 
      diet manipulation.
PG  - E25-31
AB  - Changes in dietary macronutrient intake alter muscle and blood substrate 
      availability and are important for regulating gene expression. However, few 
      studies have examined the effects of diet manipulation on gene expression in 
      human skeletal muscle. The aim of this study was to quantify the extent to which 
      altering substrate availability impacts on subsequent mRNA abundance of a subset 
      of carbohydrate (CHO)- and fat-related genes. Seven subjects consumed either a 
      low- (LOW; 0.7 g/kg body mass CHO) or high- (HIGH; 10 g/kg body mass CHO) CHO 
      diet for 48 h after performing an exhaustive exercise bout to deplete muscle 
      glycogen stores. After intervention, resting muscle and blood samples were taken. 
      Muscle was analyzed for the gene abundances of GLUT4, glycogenin, pyruvate 
      dehydrogenase kinase-4 (PDK-4), fatty acid translocase (FAT/CD36), carnitine 
      palmitoyltransferase I (CPT I), hormone-sensitive lipase (HSL), 
      beta-hydroxyacyl-CoA dehydrogenase (beta-HAD), and uncoupling binding protein-3 
      (UCP3), and blood samples for glucose, insulin, and free fatty acid (FFA) 
      concentrations. Glycogen-depleting exercise and HIGH-CHO resulted in a 300% 
      increase in muscle glycogen content (P < 0.001) relative to the LOW-CHO 
      condition. FFA concentrations were twofold higher after LOW- vs. HIGH-CHO (P < 
      0.05). The exercise-diet manipulation exerted a significant effect on 
      transcription of all carbohydrate-related genes, with an increase in GLUT4 and 
      glycogenin mRNA abundance and a reduction in PDK-4 transcription after HIGH-CHO 
      (all P < 0.05). FAT/CD36 (P < 0.05) and UCP3 (P < 0.01) gene transcriptions were 
      increased following LOW-CHO. We conclude that 1) there was a rapid capacity for a 
      short-term exercise and diet intervention to exert coordinated changes in the 
      mRNA transcription of metabolic related genes, and 2) genes involved in glucose 
      regulation are increased following a high-carbohydrate diet.
FAU - Arkinstall, Melissa J
AU  - Arkinstall MJ
AD  - School of Medical Sciences, RMIT University, PO Box 71, Bundoora, Victoria 3083, 
      Australia.
FAU - Tunstall, Rebecca J
AU  - Tunstall RJ
FAU - Cameron-Smith, David
AU  - Cameron-Smith D
FAU - Hawley, John A
AU  - Hawley JA
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20040203
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Adult
MH  - Blood Glucose/*analysis
MH  - Dietary Carbohydrates/administration & dosage/*metabolism
MH  - Exercise Tolerance/*physiology
MH  - Fatty Acids, Nonesterified/*blood
MH  - Gene Expression Regulation/*physiology
MH  - Glycogen/*metabolism
MH  - Humans
MH  - Insulin/*blood
MH  - Male
MH  - Muscle Fatigue/physiology
MH  - Muscle, Skeletal/*physiology
MH  - RNA, Messenger/metabolism
EDAT- 2004/02/06 05:00
MHDA- 2004/07/23 05:00
CRDT- 2004/02/06 05:00
PHST- 2004/02/06 05:00 [pubmed]
PHST- 2004/07/23 05:00 [medline]
PHST- 2004/02/06 05:00 [entrez]
AID - 00557.2003 [pii]
AID - 10.1152/ajpendo.00557.2003 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2004 Jul;287(1):E25-31. doi: 
      10.1152/ajpendo.00557.2003. Epub 2004 Feb 3.