PMID- 14763930
OWN - NLM
STAT- MEDLINE
DCOM- 20040525
LR  - 20190823
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 59
IP  - 2
DP  - 2004 Feb
TI  - IgE-binding components of cultured human keratinocytes in atopic 
      eczema/dermatitis syndrome and their crossreactivity with Malassezia furfur.
PG  - 168-73
AB  - BACKGROUND: Atopic eczema/dermatitis syndrome (AEDS) patients display 
      immunoglobulin E (IgE) reactivity to several antigens, e.g. saprophytic yeasts as 
      Malassezia furfur. AEDS patients also show IgE autoreactivity towards cells of 
      their own tissue including epidermis. PURPOSE OF THE STUDY: The aim of this study 
      was to investigate the IgE autoreactivity of AEDS patients to cultured 
      keratinocytes and to reveal potential crossreacting epitopes in cultured 
      keratinocytes and M. furfur. MATERIAL AND METHODS: Serum samples of 27 AEDS 
      patients were analyzed, of these 13 were M. furfur radioallergosorbent test 
      (RAST) positive and 14 negative. Four urticaria, three psoriasis, and seven 
      nonatopic patients were included as controls. The studies were performed by using 
      IgE immunoblotting and immunoblotting inhibition methods. RESULTS: Ten 
      IgE-binding protein bands were detected in cultured human keratinocytes by IgE 
      immunoblotting using sera from adult AEDS patients. Anti-keratinocyte IgE 
      antibodies were more associated with elevated S-IgE level than M. furfur RAST. 
      Clear crossreactivity with M. furfur could not be shown. CONCLUSIONS: The 
      possible pathomechanism of anti-keratinocyte IgE antibodies is not due to IgE 
      epitope mimicry of saprophytic yeast and local tissue in AEDS skin.
FAU - Kortekangas-Savolainen, O
AU  - Kortekangas-Savolainen O
AD  - Departments of Dermatology Medical Biochemistry Clinical Allergology, University 
      of Turku, Turku, Finland.
FAU - Peltonen, S
AU  - Peltonen S
FAU - Pummi, K
AU  - Pummi K
FAU - Kalimo, K
AU  - Kalimo K
FAU - Savolainen, J
AU  - Savolainen J
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Antigens, Fungal)
RN  - 0 (Autoantibodies)
RN  - 0 (Epitopes)
RN  - 0 (Galectin 3)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - *Antibody Specificity
MH  - Antigens, Fungal/immunology
MH  - Autoantibodies/analysis
MH  - Cells, Cultured
MH  - Cross Reactions
MH  - Dermatitis, Atopic/*immunology
MH  - Epitopes/immunology
MH  - Female
MH  - Galectin 3/analysis
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/immunology/*metabolism
MH  - Keratinocytes/*immunology
MH  - Malassezia/*immunology
MH  - Male
MH  - Middle Aged
MH  - Psoriasis/immunology
MH  - Radioallergosorbent Test
MH  - Urticaria/immunology
EDAT- 2004/02/07 05:00
MHDA- 2004/05/27 05:00
CRDT- 2004/02/07 05:00
PHST- 2004/02/07 05:00 [pubmed]
PHST- 2004/05/27 05:00 [medline]
PHST- 2004/02/07 05:00 [entrez]
AID - 330 [pii]
AID - 10.1046/j.1398-9995.2003.00330.x [doi]
PST - ppublish
SO  - Allergy. 2004 Feb;59(2):168-73. doi: 10.1046/j.1398-9995.2003.00330.x.