PMID- 14764000
OWN - NLM
STAT- MEDLINE
DCOM- 20040415
LR  - 20190922
IS  - 0953-8194 (Print)
IS  - 0953-8194 (Linking)
VI  - 16
IP  - 2
DP  - 2004 Feb
TI  - Regulation of galanin receptor GalR1 mRNA expression by ovarian steroids in 
      oestrogen receptor alpha-immunoreactive neurones: identification of distinct 
      populations of neurones in the preoptic area.
PG  - 138-45
AB  - This study examined whether gonadal steroids are involved in regulating galanin 
      receptor 1 (GalR1) mRNA expression in neurones that contain oestrogen receptor 
      alpha (ERalpha), in three regions of the preoptic area (POA) known to be involved 
      in the control of gonadotropin secretion. Double-labelling immunohistochemistry 
      using an antibody against the ERalpha and in situ hybridization experiments using 
      a 35S-labelled riboprobe specific for GalR1 mRNAs revealed that ERalpha is 
      expressed in a large proportion of GalR1 mRNA-expressing neurones of the POA in 
      the ovariectomized (OVX) female rat. Oestradiol (E2) and oestradiol plus 
      progesterone (E2 + P) treatments of OVX rats significantly decreased the 
      proportion of GalR1 mRNA/ERalpha immunoreactive (ERalpha-IR) neurones in the 
      anteroventral periventricular nucleus (AVPV), medial preoptic nucleus (MPN) and 
      medial preoptic area (MPO). The expression of GalR1 mRNA in ERalpha-IR neurones 
      varied according the hormonal status of the female animals. In the AVPV, during 
      the oestrous cycle, the hybridization signal significantly increased at oestrus. 
      E2 and E2 + P treatments of OVX rats did not induced significant variation of 
      levels of GalR1 mRNAs in ERalpha-IR neurones. In the MPN, E2 treatment of OVX 
      rats resulted in significant increase in GalR1 mRNA expression in ERalpha-IR 
      neurones. Similarly, levels of the GalR1 hybridization signal increased during 
      afternoon of proestrus and oestrus. In the MPO, treatment of OVX rats with E2 + P 
      significantly decreased GalR1 mRNA expression in ERalpha-IR neurones. The 
      expression of GalR1 mRNA did not change during the oestrous cycle in this area. 
      These findings suggest that the hypothalamic action of galanin on 
      gonadotopin-releasing hormone (GnRH) secretion may pass through the specific 
      population of GalR1/ERalpha-IR neurones of the MPN in mediating the oestrogen 
      action on the GnRH system at the moment of the luteinizing hormone surge.
FAU - Mitchell, V
AU  - Mitchell V
AD  - INSERM U422, Neuroendocrinology and Neuronal Physiopathology, Lille, France. 
      mitchell@lille.inserm.fr
FAU - Lecompte, F
AU  - Lecompte F
FAU - Beauvillain, J C
AU  - Beauvillain JC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Galanin, Type 1)
RN  - 0 (Receptors, Estrogen)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Estradiol/*physiology
MH  - Estrogen Receptor alpha
MH  - Estrous Cycle/physiology
MH  - Female
MH  - Gonadotropin-Releasing Hormone/physiology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Ovariectomy
MH  - Preoptic Area/*metabolism
MH  - Progesterone/*physiology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Galanin, Type 1/genetics/*metabolism
MH  - Receptors, Estrogen/genetics/*metabolism
EDAT- 2004/02/07 05:00
MHDA- 2004/04/16 05:00
CRDT- 2004/02/07 05:00
PHST- 2004/02/07 05:00 [pubmed]
PHST- 2004/04/16 05:00 [medline]
PHST- 2004/02/07 05:00 [entrez]
AID - 1147 [pii]
AID - 10.1111/j.0953-8194.2004.01147.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2004 Feb;16(2):138-45. doi: 10.1111/j.0953-8194.2004.01147.x.