PMID- 1476606
OWN - NLM
STAT- MEDLINE
DCOM- 19930205
LR  - 20191021
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 9
IP  - 3
DP  - 1992 Dec
TI  - Transforming growth factor-beta 1 production in porcine thyroid follicular cells: 
      regulation by intrathyroidal organic iodine.
PG  - 197-205
AB  - The present studies have demonstrated the production of transforming growth 
      factor-beta 1 (TGF-beta 1) by porcine thyroid follicular cells (TFCs) maintained 
      in vitro as subconfluent monolayers, and have confirmed a stimulatory effect of 
      iodide on thyroidal TGF-beta 1 mRNA and peptide release. RNA extracted from TFCs 
      maintained in the absence of iodide contained a 2.5 kb transcript which 
      hybridized specifically with a cDNA probe for human TGF-beta 1, and which showed 
      an approximate doubling in intensity in cells exposed to 10 mumol NaI/1. In the 
      presence of the anti-thyroid thionamide drug methimazole (MMI; 1 mmol/l), the 
      action of iodide on TGF-beta 1 mRNA was attenuated, although MMI alone had no 
      effect on the control level of TGF-beta 1 mRNA. The TGF-beta 1 peptide content of 
      TFC-conditioned media (TFC-CM) was assessed using the fetal mink lung cell line 
      Mv1Lu, in which activated TGF-beta 1 specifically suppresses trichloroacetic 
      acid-precipitable [methyl-3H]thymidine incorporation. Newly conditioned TFC-CM 
      stimulated [methyl-3H]thymidine incorporation into Mv1Lu cells, but after heat 
      treatment to inactivate growth stimulators and activate the latent TGF-beta 1 
      component this medium inhibited [methyl-3H]thymidine incorporation. This 
      inhibitory effect was prevented by immunoadsorption of TFC-CM with a TGF-beta 
      1-neutralizing antiserum, confirming the specificity of the inhibitory response. 
      The inhibitory activity of TFC-CM was increased when the TFCs were preincubated 
      with 10 mumol NaI/1, and lost when TFCs were exposed to MMI. In conclusion, TFCs 
      produce TGF-beta 1 mRNA and TGF-beta 1 peptide, which are both increased by 
      iodide treatment in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Cowin, A J
AU  - Cowin AJ
AD  - Department of Medicine, University of Manchester, U.K.
FAU - Davis, J R
AU  - Davis JR
FAU - Bidey, S P
AU  - Bidey SP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 554Z48XN5E (Methimazole)
RN  - 9679TC07X4 (Iodine)
SB  - IM
MH  - Animals
MH  - Culture Media, Conditioned
MH  - In Vitro Techniques
MH  - Iodine/*metabolism
MH  - Methimazole/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Swine
MH  - Thyroid Gland/cytology/drug effects/*metabolism
MH  - Transforming Growth Factor beta/*biosynthesis/genetics
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1677/jme.0.0090197 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 1992 Dec;9(3):197-205. doi: 10.1677/jme.0.0090197.