PMID- 14766605
OWN - NLM
STAT- MEDLINE
DCOM- 20040604
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 70
IP  - 2
DP  - 2004 Feb
TI  - Two bacteria phylotypes are predominant in the Suiyo seamount hydrothermal plume.
PG  - 1190-8
AB  - Microbial diversity and populations in a hydrothermal plume that was present 
      inside the caldera of the Suiyo Seamount, a submarine volcano on the Izu-Bonin 
      Arc, were investigated by performing a phylogenetic analysis of the 16S rRNA gene 
      and by using fluorescence in situ hybridization (FISH). Corresponding to 
      transmissivity, an indicator of turbidity, the vertical total cell count as 
      determined by 4',6'-diamidino-2-phenylindole (DAPI) staining varied from 5.6 x 
      10(4) to 1.1 x 10(5) cells ml(-1), and the apparent plume layer was assessed to 
      be at a depth of 1,050 to 1,200 m inside the caldera and to contain 1.0 x 10(5) 
      to 1.1 x 10(5) cells ml(-1). From microbial samples collected in the plume by an 
      in situ filtration system, the following two major phylogenetic groups, which 
      were closely related to sulfur-oxidizing microbes, were obtained: the SUP05 group 
      belonging to the gamma subclass of the Proteobacteria (13 of 20 clones) and the 
      SUP01 group belonging to the epsilon subclass of the Proteobacteria (5 of 20 
      clones). Specific oligonucleotide probes for these groups (SUP05-187 and 
      SUP01-63) were designed and were used with various water samples obtained from 
      the Suiyo Seamount. In the apparent plume layer, up to 66% of the total counts of 
      microbial cells were estimated to be Bacteria cells that hybridized to EUB338, 
      and few cells were identified by the archaeal probe ARCH915. Almost all Bacteria 
      cells were hard to identify with the known group-specific probes, such as ALF19, 
      GAM42a, and CF319, while 88 to 90% of the Bacteria cells hybridized with 
      SUP05-187 and >98% of them were considered members of the SUP05 and SUP01 
      populations. In a low-temperature vent fluid emitted from a bivalve-colonized 
      mound, the SUP05 cells accounted for >99% of the Bacteria cells, suggesting that 
      a portion of the plume cells originated on the surface of the seafloor at a depth 
      of about 1,380 m. From further analysis of cell morphology (i.e., cell size and 
      cell elongation index) we inferred that the SUP05 cells were active in the plume 
      layer at a depth of 1,050 to 1,200 m compared to the activity in a near-bottom 
      layer, while many elongated cells were found between these layers. These findings 
      suggest that the morphology and distribution of SUP05 cells have complex 
      relationships with hydrothermal activities and water circulation. Although growth 
      and production rates remain to be defined, we concluded that this Suiyo Seamount 
      caldera has functioned as a natural continuous incubator for these two phylotypes 
      of Bacteria in an aphotic deep-sea environment.
FAU - Sunamura, Michinari
AU  - Sunamura M
AD  - Biological Resources and Functions, National Institute of Advanced and Industrial 
      Science and Technology (AIST), Higashi, Tsukuba, Ibaraki 305 -8566, Japan.
FAU - Higashi, Yowsuke
AU  - Higashi Y
FAU - Miyako, Chiwaka
AU  - Miyako C
FAU - Ishibashi, Jun-ichiro
AU  - Ishibashi J
FAU - Maruyama, Akihiko
AU  - Maruyama A
LA  - eng
SI  - GENBANK/AB112446
SI  - GENBANK/AB112447
SI  - GENBANK/AB112448
SI  - GENBANK/AB112449
SI  - GENBANK/AB112450
SI  - GENBANK/AB112451
SI  - GENBANK/AB112452
SI  - GENBANK/AB112453
SI  - GENBANK/AB112454
SI  - GENBANK/AB112455
SI  - GENBANK/AB112456
SI  - GENBANK/AB112457
SI  - GENBANK/AB112458
SI  - GENBANK/AB112459
SI  - GENBANK/AB112460
SI  - GENBANK/AB112461
SI  - GENBANK/AB112462
SI  - GENBANK/AB112463
SI  - GENBANK/AB112464
SI  - GENBANK/AB112465
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Colony Count, Microbial
MH  - DNA, Ribosomal/analysis
MH  - *Ecosystem
MH  - Epsilonproteobacteria/classification/genetics/*growth & development
MH  - Gammaproteobacteria/classification/genetics/*growth & development
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/*microbiology
MH  - Sequence Analysis, DNA
MH  - Volcanic Eruptions
PMC - PMC348851
EDAT- 2004/02/10 05:00
MHDA- 2004/06/05 05:00
PMCR- 2004/02/01
CRDT- 2004/02/10 05:00
PHST- 2004/02/10 05:00 [pubmed]
PHST- 2004/06/05 05:00 [medline]
PHST- 2004/02/10 05:00 [entrez]
PHST- 2004/02/01 00:00 [pmc-release]
AID - 1183 [pii]
AID - 10.1128/AEM.70.2.1190-1198.2004 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2004 Feb;70(2):1190-8. doi: 
      10.1128/AEM.70.2.1190-1198.2004.