PMID- 14768346
OWN - NLM
STAT- MEDLINE
DCOM- 20040330
LR  - 20190823
IS  - 0387-5911 (Print)
IS  - 0387-5911 (Linking)
VI  - 77
IP  - 12
DP  - 2003 Dec
TI  - [Genotyping of methicillin-resistant Staphylococcus aureus isolated from 
      inpatients and medical workers in orthopaedics ward].
PG  - 1058-66
AB  - Methicillin-resistant Staphylococcus aureus (MRSA) strains were isolated from the 
      inpatients in orthopaedics ward hospitalized from March 1998 to November 2000, 
      hospital environments, medical workers and the inpatients transferred from TCC 
      (Trauma and Critical Care Center). Genotype by pulsed field gel electrophoresis 
      (PFGE) and biotype according to the production of coagulase, enterotoxin and 
      toxic-shock syndrome toxin-1 (TSST-1) were determined for the MRSA strains to 
      analyze the infection source and transmission route of the infection. Out of 673 
      S. aureus strains isolated from the inpatients, 390 strains (57.5%) were MRSA. In 
      89 medical workers in orthopaedics ward, MRSA were isolated in 23 (25.8%) and 7 
      (7.9%) workers from nasal cavity and hand, finger, respectively. In contrast, no 
      MRSA was isolated from hospital environments. Eighty MRSA strains (80%) from the 
      inpatients and 8 MRSA strains (75%) from the medical workers were shown to have 
      same biotype; coagulase II-enterotoxin C-TSST-1 (+) (II-C- (+)). MRSA strains 
      isolated from the inpatients were grouped into 24 types according to PFGE 
      patterns, and types 17 (17 strains), 12 (13 strains), 1 (8 strains), 4 (8 
      strains) and 13 (6 strains) were dominant among the MRSA strains isolated. It was 
      shown that MRSA strains with the same PFGE genotype were detected at the same 
      time in the different wards. In addition, MRSA strains isolated from medical 
      workers were all PFGE genotypes 1 and 4. MRSA strain isolated from a new 
      inpatient had a different PFGE type from the 24 kinds of genotype. These results 
      suggest that the involvement of the medical workers might be important as 
      infection source and for transmission of MRSA in hospital.
FAU - Moriwaki, Takahiro
AU  - Moriwaki T
AD  - Department of Orthopaedics, Kyorin University School of Medicine.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Kansenshogaku Zasshi
JT  - Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious 
      Diseases
JID - 0236671
SB  - IM
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Genotype
MH  - *Inpatients
MH  - *Medical Staff, Hospital
MH  - *Methicillin Resistance
MH  - *Nursing Staff, Hospital
MH  - Orthopedic Procedures
MH  - Staphylococcal Infections/transmission
MH  - Staphylococcus aureus/*classification/drug effects/isolation & purification
EDAT- 2004/02/11 05:00
MHDA- 2004/03/31 05:00
CRDT- 2004/02/11 05:00
PHST- 2004/02/11 05:00 [pubmed]
PHST- 2004/03/31 05:00 [medline]
PHST- 2004/02/11 05:00 [entrez]
AID - 10.11150/kansenshogakuzasshi1970.77.1058 [doi]
PST - ppublish
SO  - Kansenshogaku Zasshi. 2003 Dec;77(12):1058-66. doi: 
      10.11150/kansenshogakuzasshi1970.77.1058.