PMID- 14768923
OWN - NLM
STAT- MEDLINE
DCOM- 20040427
LR  - 20190917
IS  - 0265-0215 (Print)
IS  - 0265-0215 (Linking)
VI  - 21
IP  - 1
DP  - 2004 Jan
TI  - Release of inflammatory mediators in irradiated cell salvage blood and their 
      biological consequences in human beings following transfusion.
PG  - 46-52
AB  - BACKGROUND AND OBJECTIVE: Irradiation of intraoperative cell salvage blood has 
      recently been used to inactivate tumour cells before retransfusion, during cancer 
      surgery. No information is available about a potential inflammatory response of 
      the recipient to the retransfusion of irradiated intraoperative cell salvage 
      blood. This pilot study was conducted to investigate the possible release of the 
      pro-inflammatory mediators, tumour necrosis factor-alpha (TNF-alpha), 
      interleukin-1beta (IL-1beta), eotaxin and monocyte chemo-attractant protein-1 
      (MCP-1), in the serum of recipients by intraoperative retransfusion of irradiated 
      intraoperative cell salvage blood. METHODS: Nine patients undergoing 
      gynaecological cancer surgery were included in this study. Intraoperative cell 
      salvage blood was irradiated with 50 Gy and retransfused to the patient. Serum 
      and intraoperative cell salvage blood concentrations of TNF-alpha, IL-1beta, 
      eotaxin and MCP-1 were repeatedly analysed before and after retransfusion, 
      respectively before and after irradiation. RESULTS: Traces of mediators were 
      detected in intraoperative cell salvage blood but no increase due to irradiation 
      was observed. Following transfusion of intraoperative cell salvage blood, minute 
      quantities (all < 30 pg mL(-1) of mediators were detected in the serum of 
      patients. However, there was no significant upregulation compared to serum values 
      before retransfusion. CONCLUSIONS: These results provide evidence that 
      retransfusion of irradiated intraoperative cell salvage blood might represent a 
      blood-saving strategy in cancer surgery without an immunological inflammatory 
      response as shown by a lack of upregulation of inflammatory mediators.
FAU - Beck-Schimmer, B
AU  - Beck-Schimmer B
AD  - University Hospital, Institute of Anaesthesiology, Zurich, Switzerland. 
      beatrice.beck@usz.ch
FAU - Romero, B
AU  - Romero B
FAU - Booy, C
AU  - Booy C
FAU - Joch, H
AU  - Joch H
FAU - Hallers, U
AU  - Hallers U
FAU - Pasch, T
AU  - Pasch T
FAU - Spahn, D R
AU  - Spahn DR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Anaesthesiol
JT  - European journal of anaesthesiology
JID - 8411711
RN  - 0 (CCL11 protein, human)
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines, CC)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Aged
MH  - *Blood Preservation
MH  - Blood Transfusion, Autologous/*adverse effects/*methods
MH  - Chemokine CCL11
MH  - Chemokine CCL2/blood
MH  - Chemokines, CC/blood
MH  - Endothelial Cells/metabolism
MH  - Female
MH  - Gynecologic Surgical Procedures
MH  - Humans
MH  - Inflammation Mediators/*blood
MH  - Intercellular Adhesion Molecule-1/biosynthesis
MH  - Interleukin-1/blood
MH  - Leukocyte Count
MH  - Middle Aged
MH  - Radiation
MH  - Sterilization
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2004/02/11 05:00
MHDA- 2004/04/28 05:00
CRDT- 2004/02/11 05:00
PHST- 2004/02/11 05:00 [pubmed]
PHST- 2004/04/28 05:00 [medline]
PHST- 2004/02/11 05:00 [entrez]
AID - 10.1017/s0265021504001085 [doi]
PST - ppublish
SO  - Eur J Anaesthesiol. 2004 Jan;21(1):46-52. doi: 10.1017/s0265021504001085.