PMID- 14769229
OWN - NLM
STAT- MEDLINE
DCOM- 20040430
LR  - 20171116
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 39
IP  - 12
DP  - 2003 Dec
TI  - [The inhibitory effect of 8-chloroadenosine on tumor necrosis factor-induced 
      proto-oncogene expression of retinal pigment epithelial cells].
PG  - 750-2
AB  - OBJECTIVE: To investigate the inhibitory effect of 8-chloroadenodine (8-CLA) on 
      tumor necrosis factor-alpha (TNF-alpha)-induced proto-oncogene expression in 
      retinal pigment epithelial (RPE) cells. METHODS: Primary culture and subculture 
      of health bovine RPE cells were established in vitro. RPE cells were treated with 
      tumor necrosis factor TNF-alpha (13.3 mmol/L) with or without 8-CLA (16 micro 
      mol/L) for 30 minutes or 3 hours, respectively. C-fos and c-myc mRNA expression 
      in RPE cells was detected by Northern blot analysis. RESULTS: Normal RPE cells 
      expressed c-fos and c-myc mRNA at a low level. After treatment with TNF-alpha, 
      the expression of c-fos and c-myc mRNA was increased by 3.13 and 1.5 fold, 
      respectively, as compared to untreated cells. 8-CLA decreased TNF-alpha-induced 
      c-fos and c-myc mRNA expression by 25.0% and 29.0%, respectively. CONCLUSIONS: 
      8-CLA can inhibit TNF-alpha-induced c-fos and c-myc mRNA expression in RPE cells. 
      These findings may be useful for exploring new drugs for inhibiting the 
      growth-induced proliferation of RPE.
FAU - Yang, Ping
AU  - Yang P
AD  - The Third Hospital of Peking University, Peking University Eye Center, Beijing 
      100083, China.
FAU - Zhang, Hui-rong
AU  - Zhang HR
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 146-77-0 (2-Chloroadenosine)
RN  - 63231-63-0 (RNA)
RN  - PA05MFH3I7 (8-chloroadenosine)
SB  - IM
MH  - 2-Chloroadenosine/*analogs & derivatives/*pharmacology
MH  - Animals
MH  - Cattle
MH  - *Genes, fos
MH  - *Genes, myc
MH  - Immunohistochemistry
MH  - Pigment Epithelium of Eye/cytology/*drug effects/metabolism
MH  - RNA/analysis
MH  - RNA, Messenger/analysis
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2004/02/11 05:00
MHDA- 2004/05/01 05:00
CRDT- 2004/02/11 05:00
PHST- 2004/02/11 05:00 [pubmed]
PHST- 2004/05/01 05:00 [medline]
PHST- 2004/02/11 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2003 Dec;39(12):750-2.