PMID- 1480518
OWN - NLM
STAT- MEDLINE
DCOM- 19930209
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 13
IP  - 5
DP  - 1992 Sep-Oct
TI  - Guanine nucleotide regulation of VIP binding to rat peritoneal macrophage 
      membranes.
PG  - 953-5
AB  - In the present study, we have examined the effect of guanine nucleotides on VIP 
      binding to rat peritoneal macrophage membranes. Both guanosine 5'-triphosphate 
      (GTP) and its nonhydrolizable analog guanosine 5'-beta, Y-imidotriphosphate 
      [Gpp(NH)p] inhibited, in a dose-dependent manner, the VIP binding to its specific 
      binding sites. Half-maximal inhibition (IC50) was observed at 5.4 +/- 0.5 microM 
      GTP. The inhibitory effect of GTP was due to an increase of the dissociation rate 
      of peptide bound to membranes. The specificity of the binding inhibition was 
      assessed from the lack of action of the other nucleotides tested. These results 
      directly suggest the coupling of VIP binding sites with guanine nucleotide 
      binding proteins in rat peritoneal macrophage membranes.
FAU - Segura, J J
AU  - Segura JJ
AD  - Department of Medical Biochemistry and Molecular Biology, School of Medicine, 
      University of Sevilla, Spain.
FAU - Guerrero, J M
AU  - Guerrero JM
FAU - Goberna, R
AU  - Goberna R
FAU - Calvo, J R
AU  - Calvo JR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Cell Membrane/drug effects/metabolism
MH  - GTP-Binding Proteins/metabolism
MH  - Guanosine Triphosphate/*pharmacology
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Macrophages/*drug effects/metabolism
MH  - Peritoneal Cavity/cytology
MH  - Rats
MH  - Rats, Wistar
MH  - Vasoactive Intestinal Peptide/*metabolism
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
AID - 0196-9781(92)90055-8 [pii]
AID - 10.1016/0196-9781(92)90055-8 [doi]
PST - ppublish
SO  - Peptides. 1992 Sep-Oct;13(5):953-5. doi: 10.1016/0196-9781(92)90055-8.