PMID- 1482291
OWN - NLM
STAT- MEDLINE
DCOM- 19930205
LR  - 20210107
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 66
IP  - 9
DP  - 1992
TI  - Toxicological detection of selegiline and its metabolites in urine using 
      fluorescence polarization immunoassay (FPIA) and gas chromatography-mass 
      spectrometry (GC-MS) and differentiation by enantioselective GC-MS of the intake 
      of selegiline from abuse of methamphetamine or amphetamine.
PG  - 675-8
AB  - Selegiline (R(-)-N-methyl-N-(1-phenyl-2-propyl)-2-propinylamine), a selective 
      MAO-B inhibitor used as an antiparkinsonian, is excreted in urine as N-desmethyl 
      selegiline (norselegiline), R(-)-methamphetamine (R(-)-MA), R(-)-amphetamine 
      (R(-)-AM) and their conjugated p-hydroxy derivatives. We found that the 
      fluorescence polarization immunoassays (FPIA) TDx amphetamine/methamphetamine II 
      (AM/MA II) and TDx amphetamine class (AM class) lead to positive results for up 
      to 2 days after a single oral dose of 10 mg selegiline (detection limit: 0.1 
      mg/l, each). Every urine specimen from long term selegiline patients (10 mg/day) 
      showed positive TDx results during the selegiline regimen. Positive TDx results 
      were confirmed using gas chromatography-mass spectrometry (GC-MS). Selegiline 
      metabolites, particularly MA, could be detected in urine for up to 7 days after 
      intake of a single oral dose of 10 mg selegiline (detection limit: 0.01 mg/l for 
      MA and AM). Norselegiline, the only specific selegiline metabolite, was only 
      detectable for about 12 h. Moreover, norselegiline was not detected in all urine 
      samples from long term selegiline patients (10 mg/day). Since differentiation of 
      selegiline intake from MA/AM abuse by detecting norselegiline was not possible in 
      most cases, an enantioselective GC-MS procedure was developed. It allowed 
      differentiation of the enantiomers of the selegiline metabolites and thereby 
      separation of selegiline intake (only R(-)-enantiomers) from methamphetamine 
      and/or amphetamine abuse (racemates or S(+)-enantiomers). After derivatization 
      with S(-)-N-trifluoroacetyl-prolyl chloride (TPC), the two enantiomers of MA and 
      AM were each separated as diastereomers employing the routinely used achiral GC 
      capillary.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Maurer, H H
AU  - Maurer HH
AD  - Abteilung Toxikologie, Universitat des Saarlandes, Homburg, Federal Republic of 
      Germany.
FAU - Kraemer, T
AU  - Kraemer T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 2K1V7GP655 (Selegiline)
RN  - 44RAL3456C (Methamphetamine)
RN  - CK833KGX7E (Amphetamine)
SB  - IM
MH  - *Amphetamine
MH  - Fluorescence Polarization Immunoassay
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - *Methamphetamine
MH  - Selegiline/*toxicity/urine
MH  - Stereoisomerism
MH  - Substance Abuse Detection/*methods
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1007/BF01981508 [doi]
PST - ppublish
SO  - Arch Toxicol. 1992;66(9):675-8. doi: 10.1007/BF01981508.