PMID- 1482701
OWN - NLM
STAT- MEDLINE
DCOM- 19930218
LR  - 20071114
IS  - 1043-0342 (Print)
IS  - 1043-0342 (Linking)
VI  - 3
IP  - 6
DP  - 1992 Dec
TI  - Simultaneous use of two retroviral vectors in human gene marking trials: 
      feasibility and potential applications.
PG  - 619-24
AB  - Two Moloney murine leukemia virus (Mo-MLV)-based neoR retroviral vectors--LNL6 
      and G1Na--were used to transduce various human tumor-infiltrating lymphocytes 
      (TIL) populations. These groups included bulk CD(8+)- and CD(4+)-enriched TIL 
      from human renal cell carcinomas and melanomas. Transduction efficiencies 
      averaged 5% for single 4-hr supernatant infections. Integrated provirus could be 
      detected for up to 4 weeks of in vitro culture. LNL6 provirus could be 
      distinguished from G1Na provirus using specific polymerase chain reaction (PCR) 
      primers. A single neomycin phosphotransferase (neoR) gene copy could be detected 
      in 10(5) TIL. Using quantitative PCR, the relative ratio of LNL6 to G1Na copies 
      in the same sample could be determined even at low copy numbers. These 
      preclinical studies demonstrate the feasibility of using two retroviral marking 
      vectors in human gene therapy efforts.
FAU - Miller, A R
AU  - Miller AR
AD  - Division of Surgical Oncology, UCLA School of Medicine 90024.
FAU - Skotzko, M J
AU  - Skotzko MJ
FAU - Rhoades, K
AU  - Rhoades K
FAU - Belldegrun, A S
AU  - Belldegrun AS
FAU - Tso, C L
AU  - Tso CL
FAU - Kaboo, R
AU  - Kaboo R
FAU - McBride, W H
AU  - McBride WH
FAU - Jacobs, E
AU  - Jacobs E
FAU - Kohn, D B
AU  - Kohn DB
FAU - Moen, R
AU  - Moen R
AU  - et al.
LA  - eng
GR  - NCI R29 CA 50780/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hum Gene Ther
JT  - Human gene therapy
JID - 9008950
RN  - 0 (Genetic Markers)
SB  - IM
GS  - NeoR
MH  - Base Sequence
MH  - Carcinoma, Renal Cell/pathology
MH  - Cells, Cultured
MH  - Defective Viruses/*genetics/isolation & purification
MH  - Feasibility Studies
MH  - *Genetic Markers
MH  - *Genetic Vectors
MH  - Humans
MH  - Kidney Neoplasms/pathology
MH  - Lymphocytes, Tumor-Infiltrating/*microbiology
MH  - Melanoma/pathology
MH  - Molecular Sequence Data
MH  - Moloney murine leukemia virus/*genetics/isolation & purification
MH  - Polymerase Chain Reaction
MH  - Proviruses/isolation & purification
MH  - T-Lymphocyte Subsets/*microbiology
MH  - Transduction, Genetic
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1089/hum.1992.3.6-619 [doi]
PST - ppublish
SO  - Hum Gene Ther. 1992 Dec;3(6):619-24. doi: 10.1089/hum.1992.3.6-619.