PMID- 1486564
OWN - NLM
STAT- MEDLINE
DCOM- 19930225
LR  - 20190815
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 64
IP  - 2
DP  - 1992 Dec
TI  - Fluorescence in situ hybridization to determine engraftment status after murine 
      bone marrow transplant.
PG  - 145-8
AB  - The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in 
      situ hybridization (FISH) to evaluate murine hematopoietic tissues after 
      sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the 
      long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of 
      interphase cells from BM, spleen, and thymus after BMT was compared with Southern 
      blot analysis; both methods gave comparable results. Only FISH was able to 
      analyze post-BMT peripheral blood (PB) samples successfully, and provides a 
      useful method for following engraftment status in the mouse on an ongoing basis.
FAU - Hawkins, A L
AU  - Hawkins AL
AD  - Johns Hopkins Oncology Center, Baltimore, MD 21205.
FAU - Jones, R J
AU  - Jones RJ
FAU - Zehnbauer, B A
AU  - Zehnbauer BA
FAU - Zicha, M S
AU  - Zicha MS
FAU - Collector, M J
AU  - Collector MJ
FAU - Sharkis, S J
AU  - Sharkis SJ
FAU - Griffin, C A
AU  - Griffin CA
LA  - eng
GR  - 1 R01-HL46533-01/HL/NHLBI NIH HHS/United States
GR  - 5POI-CA 15396-17/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - *Bone Marrow Cells
MH  - *Bone Marrow Transplantation
MH  - DNA/analysis
MH  - DNA Probes
MH  - Female
MH  - Genetic Markers
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Mice
MH  - Transplantation, Isogeneic
MH  - Y Chromosome
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 0165-4608(92)90345-9 [pii]
AID - 10.1016/0165-4608(92)90345-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1992 Dec;64(2):145-8. doi: 10.1016/0165-4608(92)90345-9.