PMID- 1486806 OWN - NLM STAT- MEDLINE DCOM- 19930224 LR - 20180214 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 61 IP - 4 DP - 1992 TI - Location of the mouse complement factor H gene (cfh) by FISH analysis and replication R-banding. PG - 282-5 AB - A technique for replication R- and G-banding of mouse lymphocyte chromosomes was developed, and the replication R-banding pattern was analyzed. Optimal banding patterns were obtained with thymidine- and BrdU-treatment of lymphocytes in the same cell cycle. This produced replication R-band patterns that were the complete reverse of the G-band patterns on all chromosomes. Replication R-banding methods can be used in conjunction with nonisotopic, fluorescence in situ hybridization (FISH) to localize cloned probes to specific chromosomal bands on mouse chromosomes. with these methods the mouse complement factor H gene (cfh) was localized to the terminal portion of the F region of Chromosome 1. Q-banding patterns were also obtained by the replication R-banding method and may be useful for rapid identification of each chromosome. FAU - Matsuda, Y AU - Matsuda Y AD - Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan. FAU - Harada, Y N AU - Harada YN FAU - Natsuume-Sakai, S AU - Natsuume-Sakai S FAU - Lee, K AU - Lee K FAU - Shiomi, T AU - Shiomi T FAU - Chapman, V M AU - Chapman VM LA - eng GR - GM33160/GM/NIGMS NIH HHS/United States GR - HG00277/HG/NHGRI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 RN - 0 (DNA Probes) RN - 80295-65-4 (Complement Factor H) SB - IM GS - cfh MH - Animals MH - Cells, Cultured MH - Chromosome Banding/*methods MH - Chromosome Mapping MH - Complement Factor H/*genetics MH - DNA Probes MH - *In Situ Hybridization, Fluorescence MH - Karyotyping MH - Lymphocytes MH - Male MH - Metaphase MH - Mice MH - Mice, Inbred C57BL EDAT- 1992/01/01 00:00 MHDA- 1992/01/01 00:01 CRDT- 1992/01/01 00:00 PHST- 1992/01/01 00:00 [pubmed] PHST- 1992/01/01 00:01 [medline] PHST- 1992/01/01 00:00 [entrez] AID - 10.1159/000133423 [doi] PST - ppublish SO - Cytogenet Cell Genet. 1992;61(4):282-5. doi: 10.1159/000133423.