PMID- 14871292
OWN - NLM
STAT- MEDLINE
DCOM- 20040311
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 59
IP  - 2
DP  - 2004 Feb
TI  - Proliferative and cytotoxic response of human natural killer cells exposed to 
      transporter associated with antigen-processing-deficient cells.
PG  - 159-67
AB  - In transporter associated with antigen-processing (TAP)-deficient patients 
      affected by a severe downmodulation of human leucocyte antigen class I (HLA-I) 
      molecules, natural killer (NK) cells have an increased expression of the 
      inhibitory receptor CD94/NKG2A. Focusing our attention on NK cells, we have 
      investigated the phenotype, function and proliferative response of peripheral 
      blood lymphocytes (PBLs) derived from healthy donors after coculturing with TAP 
      (T2)- or HLA-I-deficient (721.221) cell lines and their related HLA-I-expressing 
      transfectants (T3 and DT360, respectively). After 4 days, NK cells cocultured 
      with T2 cells had a threefold increased CD94 expression compared to NK cells 
      cocultured with T3. This increase was due to proliferation of the 
      CD56brightCD94bright subset. In contrast, expression of other inhibitory 
      receptors [killer cell immunoglobulin (Ig)-like receptors] was variable during 
      time and was not related to HLA-I molecules expressed by stimulating cells. 
      Similar results were obtained using HLA-I-deficient cells (721.221). The PBLs 
      cocultured for 4 days with T2 cells displayed enhanced cytotoxic responses. The 
      results suggest that CD56brightCD94bright NK cells are induced to proliferate and 
      kill in response to a TAP-deficient environment. The changes seen in the NK-cell 
      compartment were partially contributed by T lymphocytes present in the coculture. 
      These data could explain the increased CD94 expression and autoimmune 
      manifestations observed in TAP-deficient patients.
FAU - Cerboni, C
AU  - Cerboni C
AD  - Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.
FAU - Oberg, L
AU  - Oberg L
FAU - Terrazzano, G
AU  - Terrazzano G
FAU - Zappacosta, S
AU  - Zappacosta S
FAU - Carbone, E
AU  - Carbone E
FAU - Karre, K
AU  - Karre K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antigens, CD)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (KLRD1 protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily D)
RN  - 0 (transporter associated with antigen processing (TAP))
SB  - IM
MH  - ATP-Binding Cassette Transporters
MH  - Antigens, CD/immunology
MH  - Cell Division/immunology
MH  - Coculture Techniques
MH  - Cytotoxicity Tests, Immunologic
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Killer Cells, Natural/cytology/*immunology
MH  - Lectins, C-Type/immunology
MH  - Leukocytes, Mononuclear/cytology/immunology
MH  - NK Cell Lectin-Like Receptor Subfamily D
MH  - Transfection
EDAT- 2004/02/12 05:00
MHDA- 2004/03/12 05:00
CRDT- 2004/02/12 05:00
PHST- 2004/02/12 05:00 [pubmed]
PHST- 2004/03/12 05:00 [medline]
PHST- 2004/02/12 05:00 [entrez]
AID - 1375 [pii]
AID - 10.1111/j.0300-9475.2004.01375.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2004 Feb;59(2):159-67. doi: 10.1111/j.0300-9475.2004.01375.x.