PMID- 14871295
OWN - NLM
STAT- MEDLINE
DCOM- 20040311
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 59
IP  - 2
DP  - 2004 Feb
TI  - Interleukin-4 selectively inhibits interleukin-2 secretion by 
      lipopolysaccharide-activated dendritic cells.
PG  - 183-9
AB  - Dendritic cells (DCs) generated in vitro from bone marrow precursors using 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) secrete interleukin-2 
      (IL-2) upon activation, an event probably associated to the initiation of 
      adaptive immune responses. Additionally, they produce IL-12, a cytokine related 
      to T-cell polarization. To analyse the effect of IL-4 on DC differentiation and 
      function, we assessed the capacity of murine bone marrow dendritic cells (BMDCs) 
      differentiated with GM-CSF in the presence or absence of IL-4 to produce IL-2 and 
      IL-12 upon lipopolysaccharide (LPS) activation. We found that although IL-4 
      enhanced DC IL-12p70 production, it strongly impaired IL-2 secretion by BMDCs. 
      This inhibition, which depends on the presence of IL-4 during LPS activation, is 
      DC specific, as IL-4 did not affect IL-2 secretion by T cells. Interestingly, 
      inhibition of DC IL-2 production did not prevent DC priming of T lymphocytes. 
      These results illustrate a new putative role for IL-4 on the regulation of the 
      immune response and should help clarify the controversial reports on the effect 
      of IL-4 on DCs.
FAU - Sauma, D
AU  - Sauma D
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Michea, P
AU  - Michea P
FAU - Lennon-Dumenil, A-M
AU  - Lennon-Dumenil AM
FAU - Fierro, A
AU  - Fierro A
FAU - Morales, J
AU  - Morales J
FAU - Rosemblatt, M
AU  - Rosemblatt M
FAU - Bono, M R
AU  - Bono MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Interleukin-2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Messenger)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects/immunology
MH  - Coculture Techniques
MH  - Dendritic Cells/drug effects/immunology/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Interleukin-12/biosynthesis
MH  - Interleukin-2/*antagonists & inhibitors/biosynthesis/metabolism
MH  - Interleukin-4/*pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation/drug effects/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Transgenic
MH  - Protein Subunits/biosynthesis
MH  - RNA, Messenger/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/immunology
EDAT- 2004/02/12 05:00
MHDA- 2004/03/12 05:00
CRDT- 2004/02/12 05:00
PHST- 2004/02/12 05:00 [pubmed]
PHST- 2004/03/12 05:00 [medline]
PHST- 2004/02/12 05:00 [entrez]
AID - 1380 [pii]
AID - 10.1111/j.0300-9475.2004.01380.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2004 Feb;59(2):183-9. doi: 10.1111/j.0300-9475.2004.01380.x.