PMID- 1492102
OWN - NLM
STAT- MEDLINE
DCOM- 19930303
LR  - 20190912
IS  - 0952-3278 (Print)
IS  - 0952-3278 (Linking)
VI  - 47
IP  - 4
DP  - 1992 Dec
TI  - Effect of tert-butyl hydroperoxide on cyclooxygenase and lipoxygenase metabolism 
      of arachidonic acid in rabbit platelets.
PG  - 259-64
AB  - The effect of tert-butyl hydroperoxide (t-BOOH) on the formation of thromboxane 
      (TX) B2, 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and 
      12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) from exogenous arachidonic 
      acid (AA) in washed rabbit platelets was examined. t-BOOH enhanced TXB2 and HHT 
      formation at concentrations of 8 microM and below, and at 50 microM it inhibited 
      the formation, suggesting that platelet cyclooxygenase activity can be enhanced 
      or inhibited by t-BOOH depending on the concentration. t-BOOH inhibited 12-HETE 
      production in a dose-dependent manner. When the platelets were incubated with 
      12-hydroperoxy-5,8,10,14-eicosatetraenoic acid (12-HPETE) instead of AA, t-BOOH 
      failed to inhibit the conversion of 12-HPETE to 12-HETE, indicating that the 
      inhibition of 12-HETE formation by t-BOOH occurs at the lipoxygenase step. 
      Studies utilizing indomethacin (a selective cyclooxygenase inhibitor) and 
      desferrioxamine (an iron-chelating agent) revealed that the inhibitory effect of 
      t-BOOH on the lipoxygenase is not mediated through the activation of the 
      cyclooxygenase and that this effect of t-BOOH is due to the hydroperoxy moiety. 
      These results suggest that hydroperoxides play an important role in the control 
      of platelet cyclooxygenase and lipoxygenase activities.
FAU - Fujimoto, Y
AU  - Fujimoto Y
AD  - Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, 
      Japan.
FAU - Takai, S
AU  - Takai S
FAU - Matsuno, K
AU  - Matsuno K
FAU - Sumiya, T
AU  - Sumiya T
FAU - Nishida, H
AU  - Nishida H
FAU - Sakuma, S
AU  - Sakuma S
FAU - Fujita, T
AU  - Fujita T
LA  - eng
PT  - Journal Article
PL  - Scotland
TA  - Prostaglandins Leukot Essent Fatty Acids
JT  - Prostaglandins, leukotrienes, and essential fatty acids
JID - 8802730
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Ferrous Compounds)
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 0 (Leukotrienes)
RN  - 0 (Oxidants)
RN  - 0 (Peroxides)
RN  - 0 (Platelet Aggregation Inhibitors)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 50683-78-8 (12-hydroxy-5,8,10-heptadecatrienoic acid)
RN  - 54397-85-2 (Thromboxane B2)
RN  - 59985-28-3 (12-Hydroxy-5,8,10,14-eicosatetraenoic Acid)
RN  - 67675-13-2 (12-HPETE)
RN  - 955VYL842B (tert-Butylhydroperoxide)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 1.13.11.12 (Lipoxygenase)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
RN  - J06Y7MXW4D (Deferoxamine)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
MH  - Animals
MH  - Arachidonic Acid/*blood
MH  - Blood Platelets/drug effects/*enzymology/*metabolism
MH  - Deferoxamine/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Fatty Acids, Unsaturated/biosynthesis
MH  - Ferrous Compounds/pharmacology
MH  - Hydroxyeicosatetraenoic Acids/biosynthesis
MH  - Indomethacin/pharmacology
MH  - Leukotrienes/pharmacology
MH  - Lipoxygenase/*blood
MH  - Male
MH  - Oxidants/*pharmacology
MH  - Peroxides/*pharmacology
MH  - Platelet Aggregation Inhibitors/pharmacology
MH  - Prostaglandin-Endoperoxide Synthases/*blood
MH  - Quercetin/pharmacology
MH  - Rabbits
MH  - Thromboxane B2/biosynthesis
MH  - tert-Butylhydroperoxide
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1016/0952-3278(92)90195-o [doi]
PST - ppublish
SO  - Prostaglandins Leukot Essent Fatty Acids. 1992 Dec;47(4):259-64. doi: 
      10.1016/0952-3278(92)90195-o.