PMID- 1492788
OWN - NLM
STAT- MEDLINE
DCOM- 19930301
LR  - 20031114
IS  - 0021-4884 (Print)
IS  - 0021-4884 (Linking)
VI  - 41
IP  - 11
DP  - 1992 Nov
TI  - Eosinophil infiltration and enhancement of airway reactivity by leukocyte 
      chemotactic factor, formyl-methionyl-leucyl-phenylalanine (fMLP), in guinea pigs.
PG  - 1547-60
AB  - N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) is a bacterial-derived 
      chemotactic factor for eosinophils and neutrophils. This study is aimed to 
      examine whether or not eosinophil infiltration induced by intra-airway 
      administration of fMLP causes the damage of the bronchial epithelium and results 
      in airway hyperresponsiveness in normal non-sensitized guinea pigs. In normal 
      guinea pigs fMLP administered by aerosol inhalation or intratracheal injection 
      caused significant infiltration of eosinophils in the tracheal mucosa and 
      enhanced bronchial reactivity to inhaled histamine 6 and 24 hours after exposure. 
      Electron microscopic examination showed damage of the alignment of the epithelial 
      cells in the bronchial mucosa in fMLP-treated guinea pigs. PAF antagonists CV3988 
      and WEB2086 and a 5-lipoxygenase inhibitor (AA-861) did not prevent fMLP induced 
      eosinophil infiltration, which suggests that fMLP caused eosinophil infiltration 
      mainly by its chemotactic activity, not by the release of platelet activating 
      factor (PAF) or leukotrienes in this experimental condition. These results showed 
      that in normal guinea pigs a bacteria-derived chemoattractant of fMLP could 
      reproduce a sequence of eosinophil infiltration and airway hyperresponsiveness, 
      similar to the inflammatory pathophysiology after antigen challenge in sensitized 
      animals. We concluded that eosinophil infiltration induced by either 
      immunological or non-immunological mechanisms can cause airway damage and airway 
      hyperresponsiveness.
FAU - Amagai, M
AU  - Amagai M
AD  - Department of Medicine and Clinical Immunology, Dokkyo University School of 
      Medicine.
FAU - Ohashi, Y
AU  - Ohashi Y
FAU - Makino, S
AU  - Makino S
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Arerugi
JT  - Arerugi = [Allergy]
JID - 0241212
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Administration, Inhalation
MH  - Animals
MH  - Bronchial Hyperreactivity/*immunology/pathology
MH  - Eosinophils/drug effects/*immunology
MH  - Guinea Pigs
MH  - Lung/drug effects/immunology
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/administration & dosage/*pharmacology
MH  - Trachea/ultrastructure
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
PST - ppublish
SO  - Arerugi. 1992 Nov;41(11):1547-60.