PMID- 1493048
OWN - NLM
STAT- MEDLINE
DCOM- 19930309
LR  - 20131121
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 8
IP  - 12
DP  - 1992 Dec
TI  - Regulatory elements in the human immunodeficiency virus type 1 long terminal 
      repeat LTR (HIV-1) responsive to steroid hormone stimulation.
PG  - 1977-80
AB  - Within the long terminal repeat (LTR) of the human immunodeficiency virus type 1 
      (HIV-1) provirus there exists a steroid hormone-responsive element corresponding 
      to the TGTTCT sequence identified as the glucocorticoid receptor binding element 
      within the LTR of mouse mammary tumor virus. We have used an 
      LTR(HIV-1)-chloramphenicol acetyl transferase (CAT) plasmid construct to 
      transfect infected H9V3 and noninfected H9 cells. Four hours before harvest the 
      cells were divided into two parts and half was treated with hydrocortisone 
      (10(-7) M). The cells were harvested and washed, and the CAT activity was 
      measured. In eight repeat experiments an increased expression of the CAT gene has 
      consistently been observed in H9V3 cells in response to the glucocorticoid but no 
      significant effect of the steroid was observed in noninfected cells. Double 
      transfection of LTR(HIV-1)-TAT and LTR(HIV-1)-CAT into noninfected H9 cells 
      results in a cell population in which the CAT gene was responsive to 
      glucocorticoid stimulation. A time course and dose response for the steroid 
      effect have been determined and the binding of steroid receptor fo the LTR-DNA 
      characterized by gel retardation experiments.
FAU - Kolesnitchenko, V
AU  - Kolesnitchenko V
AD  - Departement de Physiologie Cellulaire, Universite Pierre et Marie Curie, Paris, 
      France.
FAU - Snart, R S
AU  - Snart RS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (DNA, Viral)
RN  - 0 (Receptors, Steroid)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
CIN - AIDS Res Hum Retroviruses. 1993 Nov;9(11):1055-6. PMID: 8312048
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Viral/genetics
MH  - Genes, Regulator/drug effects
MH  - Genes, Viral/drug effects
MH  - HIV Long Terminal Repeat/*drug effects/genetics
MH  - HIV-1/drug effects/genetics
MH  - Humans
MH  - Hydrocortisone/*pharmacology
MH  - Receptors, Steroid/drug effects/metabolism
MH  - Transfection
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1089/aid.1992.8.1977 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1992 Dec;8(12):1977-80. doi: 10.1089/aid.1992.8.1977.