PMID- 1493810
OWN - NLM
STAT- MEDLINE
DCOM- 19930311
LR  - 20131121
IS  - 0171-9335 (Print)
IS  - 0171-9335 (Linking)
VI  - 59
IP  - 2
DP  - 1992 Dec
TI  - Interleukin-1 is a motility factor for human breast carcinoma cells in vitro: 
      additive effect with interleukin-6.
PG  - 449-57
AB  - Interleukin-1 beta (Il-1 beta) and interleukin-1 alpha (Il-1 alpha) were shown to 
      act as motility factors for the human breast carcinoma cell lines SK-BR-3 and 
      ZR-75-1 in vitro. Both cytokines induced transition from the stationary to the 
      motile phenotype (spreading). Il-1 beta stimulated translocation, shape change 
      and random migration (chemokinesis) of SK-BR-3 cells as demonstrated by 
      time-lapse video recordings and by a modified Boyden chamber assay. Interleukin-6 
      (Il-6) stimulated spreading of the SK-BR-3 cells; an additive effect with Il-1 
      beta on spreading and fast plasma membrane movements was evidenced. In the 
      SK-BR-3 cell line, the signal transduction of Il-1 beta and Il-6 differed, since 
      only the effect of Il-6 on spreading was sensitive to pertussis toxin. Both Il-1 
      beta and Il-6 required protein synthesis to stimulate spreading, since 
      cycloheximide inhibited the effect of the cytokines. Induction of an autocrine 
      loop of Il-6 in the SK-BR-3 cells by Il-1 beta was unlikely, since after 
      stimulation with Il-1 beta, no induction of Il-6 activity was measured, nor was 
      inhibition of stimulated spreading seen in the presence of an antiserum against 
      Il-6. Addition of Il-8 or of an antiserum against Il-8 did not affect spreading. 
      We concluded that Il-1 and Il-6 could act as motility factors for human breast 
      carcinoma cells, in both an independent and an additive way.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Verhasselt, B
AU  - Verhasselt B
AD  - Department of Radiotherapy and Nuclear Medicine, University Hospital, 
      Gent/Belgium.
FAU - Van Damme, J
AU  - Van Damme J
FAU - van Larebeke, N
AU  - van Larebeke N
FAU - Put, W
AU  - Put W
FAU - Bracke, M
AU  - Bracke M
FAU - De Potter, C
AU  - De Potter C
FAU - Mareel, M
AU  - Mareel M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 2.4.2.31 (Pertussis Toxin)
SB  - IM
MH  - Breast Neoplasms/*pathology
MH  - Cell Membrane/drug effects
MH  - Cell Movement/drug effects
MH  - Cell Size/drug effects
MH  - Culture Media, Conditioned
MH  - Cycloheximide/pharmacology
MH  - Drug Interactions
MH  - Female
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Interleukin-1/*pharmacology
MH  - Interleukin-6/*pharmacology
MH  - Pertussis Toxin
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Cells, Cultured
MH  - Video Recording
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
PST - ppublish
SO  - Eur J Cell Biol. 1992 Dec;59(2):449-57.