PMID- 14960310
OWN - NLM
STAT- MEDLINE
DCOM- 20040326
LR  - 20181130
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 559
IP  - 1-3
DP  - 2004 Feb 13
TI  - Interleukin-12 secreted by mature dendritic cells mediates activation of NK cell 
      function.
PG  - 71-6
AB  - Dendritic cells (DCs) are known to modulate immune response by activating 
      effector cells of both the innate and the adaptive immune system. In the present 
      study, we demonstrate that co-culture of DCs with paraformaldehyde-fixed tumor 
      cells augments the secretion of interleukin (IL)-12 by DCs and these activated 
      DCs upon co-culture with naive NK cells enhance the cytolytic activity of NK 
      cells against NK-sensitive target YAC-1. Similarly, DCs isolated from 
      tumor-bearing animals also activated NK cells in vitro. For efficient activation 
      of NK cells, the ratio of activated DCs to NK cells is crucial. Addition of 
      anti-IL-12 antibody to the culture system completely abolished activation of NK 
      cells by DCs, suggesting that IL-12 secreted by DCs is an essential factor in NK 
      cell activation. Adoptive transfer of DCs isolated from tumor-bearing animals 
      into normal rats also induced activation of NK cells in normal animals.
FAU - Alli, Rajshekhar S
AU  - Alli RS
AD  - Centre for Cellular and Molecular Biology, Uppal Road, Hyderabd 500 007, India.
FAU - Khar, Ashok
AU  - Khar A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Coculture Techniques
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/*immunology/metabolism/transplantation
MH  - Interleukin-12/metabolism/*physiology
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation/immunology
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Fixation
MH  - Tumor Cells, Cultured
EDAT- 2004/02/13 05:00
MHDA- 2004/03/27 05:00
CRDT- 2004/02/13 05:00
PHST- 2003/11/05 00:00 [received]
PHST- 2003/12/15 00:00 [revised]
PHST- 2003/12/29 00:00 [accepted]
PHST- 2004/02/13 05:00 [pubmed]
PHST- 2004/03/27 05:00 [medline]
PHST- 2004/02/13 05:00 [entrez]
AID - S0014579304000262 [pii]
AID - 10.1016/S0014-5793(04)00026-2 [doi]
PST - ppublish
SO  - FEBS Lett. 2004 Feb 13;559(1-3):71-6. doi: 10.1016/S0014-5793(04)00026-2.