PMID- 14960326
OWN - NLM
STAT- MEDLINE
DCOM- 20040326
LR  - 20171116
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 559
IP  - 1-3
DP  - 2004 Feb 13
TI  - Coactivator ASC-2 mediates heat shock factor 1-mediated transactivation dependent 
      on heat shock.
PG  - 165-70
AB  - Upon exposure to elevated temperatures, mammalian cells increase the expression 
      of the heat shock proteins (HSP) through activation of the heat shock factor 1 
      (HSF1). Since most transcription factors require coactivators for efficient 
      transcriptional activity, we tried to identify the coactivator(s) that interacts 
      with and modulates the activities of HSF1. In vitro glutathione S-transferase 
      (GST) pull-down assay revealed that HSF1 strongly interacts with activating 
      signal cointegrator (ASC)-2 and weakly with cyclic adenosine monophosphate 
      responsive element binding protein (CBP). We also show that cotransfection of 
      ASC-2, but not CBP, potentiates HSF1-mediated transactivation based on its 
      cognate element (heat shock element, HSE) linked to luciferase reporter. The 
      molecular interaction of HSF1 and ASC-2 was stimulated by heat shock in cells and 
      the overexpression of HSF1-interacting domain of ASC-2 inhibited the specific 
      induced protein association and HSF1-mediated transactivation. Taking these 
      results together, we suggest that ASC-2 in a novel coactivator for HSF1 and heat 
      shock stress may contribute the strong active transcription complex through 
      sequential recruitment of HSF1 and ASC-2.
FAU - Hong, SunHwa
AU  - Hong S
AD  - Department of Molecular Biology, Pusan National University, Busan 609-735, South 
      Korea.
FAU - Kim, Sun Hee
AU  - Kim SH
FAU - Heo, Mi Ae
AU  - Heo MA
FAU - Choi, Yoon Ha
AU  - Choi YH
FAU - Park, Min Jung
AU  - Park MJ
FAU - Yoo, Mi Ae
AU  - Yoo MA
FAU - Kim, Han Do
AU  - Kim HD
FAU - Kang, Ho Sung
AU  - Kang HS
FAU - Cheong, JaeHun
AU  - Cheong J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HSF1 protein, human)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (NCOA6 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nuclear Receptor Coactivators)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (CREBBP protein, human)
SB  - IM
MH  - Binding Sites
MH  - CREB-Binding Protein
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/metabolism/*physiology
MH  - Heat Shock Transcription Factors
MH  - Heat Stress Disorders/*genetics
MH  - Humans
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Luciferases/genetics
MH  - Nuclear Proteins/metabolism/physiology
MH  - Nuclear Receptor Coactivators
MH  - Protein Binding
MH  - Protein Interaction Mapping
MH  - Trans-Activators/metabolism/physiology
MH  - Transcription Factors/metabolism/*physiology
MH  - *Transcriptional Activation
EDAT- 2004/02/13 05:00
MHDA- 2004/03/27 05:00
CRDT- 2004/02/13 05:00
PHST- 2003/08/06 00:00 [received]
PHST- 2003/10/07 00:00 [revised]
PHST- 2003/10/17 00:00 [accepted]
PHST- 2004/02/13 05:00 [pubmed]
PHST- 2004/03/27 05:00 [medline]
PHST- 2004/02/13 05:00 [entrez]
AID - S0014579304000286 [pii]
AID - 10.1016/S0014-5793(04)00028-6 [doi]
PST - ppublish
SO  - FEBS Lett. 2004 Feb 13;559(1-3):165-70. doi: 10.1016/S0014-5793(04)00028-6.