PMID- 14960711
OWN - NLM
STAT- MEDLINE
DCOM- 20040513
LR  - 20181113
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 32
IP  - 3
DP  - 2004 Feb 11
TI  - Semiconductor nanocrystal probes for human metaphase chromosomes.
PG  - e28
AB  - To improve signal stability and quantitation, an optically stable, novel class of 
      fluorophore for hybridization analysis of human metaphase chromosomes is 
      demonstrated. Detection of hybridization sites in situ was based on fluorescence 
      from streptavidin-linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. 
      Fluorescence of nanocrystal fluorophores was significantly brighter and more 
      photostable than organic fluorophores Texas Red and fluorescein. Thus, 
      semiconductor nanocrystal fluorophores offer a more stable and quantitative mode 
      of fluorescence in situ hybridization (FISH) for research and clinical 
      applications.
FAU - Xiao, Yan
AU  - Xiao Y
AD  - GeoCenters, Inc. and DNA Technologies Group, National Institute of Standards and 
      Technology, Gaithersburg, MD 20899-8311, USA .
FAU - Barker, Peter E
AU  - Barker PE
LA  - eng
SI  - PDB/1Q12
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040211
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Cadmium Compounds)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Selenium Compounds)
RN  - 9007-49-2 (DNA)
RN  - 9013-20-1 (Streptavidin)
RN  - A7F646JC5C (cadmium selenide)
SB  - IM
MH  - Biotinylation
MH  - Cadmium Compounds/*chemistry
MH  - Cell Line, Tumor
MH  - Chromosome Mapping
MH  - Chromosomes, Human/*genetics
MH  - Crystallization
MH  - DNA/chemistry/*genetics
MH  - Fluorescent Dyes/chemistry
MH  - Genes, erbB-2/genetics
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Metaphase/genetics
MH  - Nanotechnology/methods
MH  - Selenium Compounds/*chemistry
MH  - Sensitivity and Specificity
MH  - Streptavidin/chemistry
PMC - PMC373420
EDAT- 2004/02/13 05:00
MHDA- 2004/05/14 05:00
PMCR- 2004/02/11
CRDT- 2004/02/13 05:00
PHST- 2004/02/13 05:00 [pubmed]
PHST- 2004/05/14 05:00 [medline]
PHST- 2004/02/13 05:00 [entrez]
PHST- 2004/02/11 00:00 [pmc-release]
AID - 32/3/e28 [pii]
AID - gnh024 [pii]
AID - 10.1093/nar/gnh024 [doi]
PST - epublish
SO  - Nucleic Acids Res. 2004 Feb 11;32(3):e28. doi: 10.1093/nar/gnh024.