PMID- 14961210
OWN - NLM
STAT- MEDLINE
DCOM- 20041223
LR  - 20220409
IS  - 0171-967X (Print)
IS  - 0171-967X (Linking)
VI  - 74
IP  - 5
DP  - 2004 May
TI  - Mechanical stimulation promotes osteogenic differentiation of human bone marrow 
      stromal cells on 3-D partially demineralized bone scaffolds in vitro.
PG  - 458-68
AB  - Bone is a dynamic tissue that is able to sense and adapt to mechanical stimuli by 
      modulating its mass, geometry, and structure. Bone marrow stromal cells (BMSCs) 
      are known to play an integral part in bone formation by providing an 
      osteoprogenitor cell source capable of differentiating into mature osteoblasts in 
      response to mechanical stresses. Characteristics of the in vivo bone environment 
      including the three dimensional (3-D) lacunocanalicular structure and 
      extracellular matrix composition have previously been shown to play major roles 
      in influencing mechanotransduction processes within bone cells. To more 
      accurately model this phenomenon in vitro, we cultured human BMSCs on 3-D, 
      partially demineralized bone scaffolds in the presence of four-point bending 
      loads within a novel bioreactor. The effect of mechanical loading and 
      dexamethasone concentration on BMSC osteogenic differentiation and mineralized 
      matrix production was studied for 8 and 16 days of culture. Mechanical 
      stimulation after 16 days with 10 nM dexamethasone promoted osteogenic 
      differentiation of BMSCs by significantly elevating alkaline phosphatase activity 
      as well as alkaline phosphatase and osteopontin transcript levels over static 
      controls. Mineralized matrix production also increased under these culture 
      conditions. Dexamethasone concentration had a dramatic effect on the ability of 
      mechanical stimulation to modulate these phenotypic and genotypic responses. 
      These results provide increased insight into the role of mechanical stimulation 
      on osteogenic differentiation of human BMSCs in vitro and may lead to improved 
      strategies in bone tissue engineering.
FAU - Mauney, J R
AU  - Mauney JR
AD  - Tufts University, Department of Biomedical Engineering, Biotechnology Center, 4 
      Colby Street, Medford, Massachusetts 02155, USA.
FAU - Sjostorm, S
AU  - Sjostorm S
FAU - Blumberg, J
AU  - Blumberg J
FAU - Horan, R
AU  - Horan R
FAU - O'Leary, J P
AU  - O'Leary JP
FAU - Vunjak-Novakovic, G
AU  - Vunjak-Novakovic G
FAU - Volloch, V
AU  - Volloch V
FAU - Kaplan, D L
AU  - Kaplan DL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20040217
PL  - United States
TA  - Calcif Tissue Int
JT  - Calcified tissue international
JID - 7905481
RN  - 0 (Glucocorticoids)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Adult
MH  - Alkaline Phosphatase/metabolism
MH  - Bone Demineralization Technique
MH  - Bone Marrow Cells/*cytology/drug effects
MH  - Bone Matrix/drug effects/physiology
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cells, Cultured
MH  - Compressive Strength/drug effects
MH  - Dexamethasone/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Glucocorticoids/pharmacology
MH  - Humans
MH  - Osteogenesis/drug effects/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stress, Mechanical
MH  - Stromal Cells/*cytology/drug effects
MH  - Tensile Strength/drug effects
MH  - Tissue Engineering/instrumentation/*methods
EDAT- 2004/02/13 05:00
MHDA- 2004/12/24 09:00
CRDT- 2004/02/13 05:00
PHST- 2003/09/21 00:00 [received]
PHST- 2003/10/03 00:00 [accepted]
PHST- 2004/02/13 05:00 [pubmed]
PHST- 2004/12/24 09:00 [medline]
PHST- 2004/02/13 05:00 [entrez]
AID - 10.1007/s00223-003-0104-7 [doi]
PST - ppublish
SO  - Calcif Tissue Int. 2004 May;74(5):458-68. doi: 10.1007/s00223-003-0104-7. Epub 
      2004 Feb 17.