PMID- 14961310 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20040226 LR - 20040212 IS - 1436-2228 (Print) IS - 1436-2228 (Linking) VI - 3 IP - Supplement 1 DP - 2001 Jun TI - Application of fluorescence in situ hybridization (FISH) to fish genetics and genome mapping. PG - S145-52 AB - The various applications of the technique of fluorescence in situ hybridization (FISH) to fish genetics will be reviewed for fishes being used as model organisms to study human disease, including those species for which major genome projects have been initiated. "FISH on fish" has been used to map highly repetitive sequences including centromere-specific sequences and sex-specific sequences, moderately repetitive sequences such as rRNAs and histones, and single-copy sequences. Paint probes specific for whole genomes, whole chromosomes, and chromosome subregions have been prepared for several species. The potential future applications of FISH to fish genetics and genome mapping will be discussed. FAU - Phillips, R B AU - Phillips RB AD - Department of Biological Sciences, University of Wisconsin-Milwaukee, Box 413, Milwaukee, WI 53201, USA. rp@csd.uwm.edu LA - eng PT - Journal Article PL - United States TA - Mar Biotechnol (NY) JT - Marine biotechnology (New York, N.Y.) JID - 100892712 EDAT- 2004/02/13 05:00 MHDA- 2004/02/13 05:01 CRDT- 2004/02/13 05:00 PHST- 2004/02/13 05:00 [pubmed] PHST- 2004/02/13 05:01 [medline] PHST- 2004/02/13 05:00 [entrez] AID - 10.1007/s10126-001-0036-z [doi] PST - ppublish SO - Mar Biotechnol (NY). 2001 Jun;3(Supplement 1):S145-52. doi: 10.1007/s10126-001-0036-z.