PMID- 14961342
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20040226
LR  - 20191108
IS  - 1436-2228 (Print)
IS  - 1436-2228 (Linking)
VI  - 3
IP  - 5
DP  - 2001 Sep
TI  - A centromeric satellite sequence in the Pacific oyster (Crassostrea gigas 
      Thunberg) identified by fluorescence in situ hybridization.
PG  - 486-92
AB  - A highly repetitive satellite sequence was previously identified in the Pacific 
      oyster Crassostrea gigas Thunberg. The sequence has 168 bp per unit, present in 
      tandem repeats, and accounts for 1% to 4% of the genome. We studied the 
      chromosomal location of this satellite sequence by fluorescence in situ 
      hybridization (FISH). A probe was made by polymerase chain reaction and 
      incorporation of digoxigenin-11-dUTP. Hybridization was detected with 
      fluorescein-labeled antidigoxigenin antibodies. FISH signals were located at 
      centromeric regions of 7 pairs of the Pacific oyster chromosomes. No interstitial 
      site was found. Signals were strong and consistent on chromosomes 1, 2, 4, and 7, 
      but weak or variable on chromosomes 5, 8, and 10. No signal was observed on 
      chromosomes 3, 6, and 9. Our results showed that this sequence is clearly a 
      centromeric satellite, disputing its previous assignment to the telomeric and 
      submetacentric regions of 2 chromosomes. No signal was detected in the American 
      oyster (Crassostrea virginica Gmelin).
FAU - Wang, Y
AU  - Wang Y
AD  - Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, 
      Rutgers University, 6959 Miller Avenue, Post Norris, NJ 08349, USA.
FAU - Xu, Z
AU  - Xu Z
FAU - Guo, X
AU  - Guo X
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
EDAT- 2004/02/13 05:00
MHDA- 2004/02/13 05:01
CRDT- 2004/02/13 05:00
PHST- 2004/02/13 05:00 [pubmed]
PHST- 2004/02/13 05:01 [medline]
PHST- 2004/02/13 05:00 [entrez]
AID - 10.1007/s10126-001-0063-3 [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2001 Sep;3(5):486-92. doi: 10.1007/s10126-001-0063-3.